Effect of diethylnitrosamine on renal metabolic pathway in rats.
10.11817/j.issn.1672-7347.2019.180518
- Author:
Dinala QIAREFUHAN
1
;
Mireayi YALIKUN
1
;
Tailiaiti TUERHONG
2
;
Akilzaman MAMAT
2
;
Batur MAMTIMIN
3
Author Information
1. College of Pharmacy, Xinjiang Medical University, Urumqi 830011, China.
2. Institute of Traditional Uyghur Medicine, Xinjiang Medical University, Urumqi 830011, China.
3. Central Laboratory, Xinjiang Medical University, Urumqi 830011, China.
- Publication Type:Journal Article
- MeSH:
Alkylating Agents;
toxicity;
Animals;
Diethylnitrosamine;
toxicity;
Glycine;
Kidney;
drug effects;
physiology;
Metabolic Networks and Pathways;
drug effects;
Rats;
Rats, Sprague-Dawley
- From:
Journal of Central South University(Medical Sciences)
2019;44(9):990-995
- CountryChina
- Language:Chinese
-
Abstract:
To explore the renal metabolic markers relavant to the renal toxicity of diethylnitrosamine and the metabolic pathways involved in the renal metabolic markers.
Methods: Nineteen Sprague Dawley rats were assigned into 2 groups: A normal control group (n=9) and a diethylnitrosamine (DEN) administration group (n=10). The rats in the normal control group were given sterilized water for free drinking. The rats in the DEN administration group were given 0.1 mg/mL DEN solution for free drinking. After 18 weeks, the kidney tissues were collected and tested for nuclear magnetic resonance detection and pathological examination.
Results: The content of kidneys metabolites in the rats with the DEN administration was changed significantly. The levels of alanine, taurine, pyruvate, acetate, and choline were significantly reduced compared with rat in the normal control group, while the levels of creatine, glycine, TMAO, methionine, proline, lactate, valine, leucine and isoleucine were significantly increased.
Conclusion: Metabolicomics studies have revealed significant differences in five metabolic pathways, including valine, leucine and isoleucine biosynthesis, glycine serine and threonine metabolism, pyruvate metabolism, glycolysis or gluconeogenesis, cysteine and methionine metabolism.
