Antiviral activity of nano-realgar against herpes simplex virus Type II in vitro.
10.11817/j.issn.1672-7347.2019.180552
- Author:
Dan WANG
1
;
Li WANG
2
;
Rui XU
2
;
Xing'an WU
3
;
Yunlan LI
1
Author Information
1. Department of Pharmaceutical Analysis, School of Pharmaceutical Science, Shanxi Medical University, Taiyuan 030001, China.
2. Research Section, First Afliated Hospital, Xi'an Medical University, Xi'an 710077, China.
3. Department of Microbiology and Pathogenic Biology, Air Force Medical University, Xi'an 710032, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antiviral Agents;
Arsenicals;
Chlorocebus aethiops;
Herpesvirus 1, Human;
Herpesvirus 2, Human;
Sulfides;
Vero Cells
- From:
Journal of Central South University(Medical Sciences)
2019;44(10):1143-1150
- CountryChina
- Language:Chinese
-
Abstract:
To explore the antiviral activity of nano-realgar against herpes simplex virus Type II (HSV-2) in vitro.
Methods: Acyclovir (ACV) as a positive control, the cytotoxicity of nano-realgar at different concentrations (including 200.00, 150.00, 100.00, 50.00, 25.00, 12.50, 6.25, 3.13, 1.54, 0.78, 0.39 and 0 mg/L) on normal Vero cells were determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. HSV-2 virus titer was determined by plaque assay, and the Vero cells model of HSV-2 infection was established. Subsequently, the antiviral effects of nano-realgar at different concentrations (including 20.00, 10.00, 5.00, 2.50, 1.25, 0.63, 0.31, 0.15, 0.08, 0.04 and 0 mg/L) on infected cells model were evaluated by the observation of cytopathic effect (CPE) and MTT method under the 3 modes including pre-treatment, treatment and direct inactivation.
Results: The 50% cytotoxic concentration (CC50) of nano-realgar on Vero cells was 37.15 mg/L. The titer of HSV-2 was 7.30 log PFUs/mL. In the 3 modes, the half-maximal effective concentration (EC50) of nano-realgar on HSV-2 infected Vero cells were 0.13, 1.80 and 0.52 mg/L, and the corresponding therapeutic index (TI) were 285.77, 20.64, 71.44, respectively. The TI value of nano-realgar on pre-treatment mode was higher than that of nano-realgar on treatment and direct inactivation modes.
Conclusion: Nano-realgar can play a good anti-HSV-2 activity in the 3 modes (pre-treatment, treatment and direct inactivation), and the anti-HSV-2 efficacy of nano-realgar on pre-treatment mode is better than that of nano-realagr on other 2 modes.
