THAP11 mediates the proliferation and apoptosis of esophageal cancer cells via inhibiting ubiquitination of p53.
10.11817/j.issn.1672-7347.2019.180642
- Author:
Yingying JIN
1
;
Baofeng WANG
2
;
Yue KE
2
;
Yi LI
2
;
Fang LI
2
;
Heng LUO
2
;
Xin MENG
2
;
Xinyue CUI
2
Author Information
1. Department of Oncological Radiotherapy, Second Hospital Affi liated to Xi'an Jiaotong University, Xi'an 710004, China yingyingjin717@sina.com.
2. Department of Oncological Radiotherapy, Second Hospital Affi liated to Xi'an Jiaotong University, Xi'an 710004, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Cell Line, Tumor;
Cell Proliferation;
Esophageal Neoplasms;
Humans;
Repressor Proteins;
metabolism;
Tumor Suppressor Protein p53;
Ubiquitination
- From:
Journal of Central South University(Medical Sciences)
2019;44(12):1338-1343
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of thanatos-associated protein 11 (THAP11) on the proliferation and apoptosis of esophageal cancer cell and the underlying mechanism.
Methods: Expression of THAP11 in human esophageal epithelial cells (Het-1A) and esophageal cancer cells (Eca109, TE-1, Ec 9706) were detected by Western blotting. Esophageal cancer TE-1 cells were divided into 3 groups: a normal control (NC) group, a negative control (LV-NC) group and a THAP11 (LV-THAP11) group. Then the cell proliferation were detected by MTT assay, cell apoptosis were detected by flow cytometry, caspase-3 and caspase-9 levels were detected by caspases kits. Ubiquitination of p53 was determined in esophageal cancer TE-1 cells.
Results: Expression of THAP11 was reduced in esophageal cancer cells compared with human esophageal epithelial cells (P<0.05). After transfection with LV-THAP11 in TE-1 cells, cell viability was reduced (P<0.05), while apoptosis rate and caspase-3 and caspase-9 levels were increased (P<0.05), indicating that THAP11 inhibited growth of esophageal cancer cells. In addition, the THAP11 increased the levels of p53 (P<0.05) and inhibited the ubiquitination of p53 regulated by MDM2.
Conclusion: THAP11 may inhibit the proliferation of esophageal cancer cells by inhibiting ubiquitination of p53.
