Effect of icariin on early βdefensin2 and T cell subsets in rats after tracheotomy.
10.11817/j.issn.1672-7347.2020.180754
- Author:
Jing JU
1
;
Renqian TAN
1
;
Yong ZHONG
1
;
Lingling PENG
2
;
Yuzheng PAN
2
Author Information
1. Department of Critical Care Medcine, Chongqing Traditional Chinese Medicine Hospital of Yongchuan District, Chongqing 404000.
2. Department of Traditional Chinese Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.
- Publication Type:Journal Article
- Keywords:
T cell subset;
early lung immune function;
icariin;
tracheotomy model;
β-defensin-2
- MeSH:
Animals;
Flavonoids;
Male;
Rats;
Rats, Sprague-Dawley;
T-Lymphocyte Subsets;
Tracheotomy;
beta-Defensins
- From:
Journal of Central South University(Medical Sciences)
2020;45(1):1-7
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To investigate the effect of icariin (ICA) on early β-defensin-2 and T cell subsets in rats after tracheotomy.
METHODS:A total of 54 SPF male Sprague-Dawley rats were randomly divided into a normal control group (group A), a model group (group B), and a model+ICA treatment group (group C), with 18 rats in each group. A tracheotomy intubation model of the B and C group was prepared. After 6 h of surgery, ICA intervention was given to group C. Groups A and B were given the same amount of normal saline. Lung tissue, alveolar lavage fluid and peripheral blood were taken at 24 h, 72 h and 168 h, respectively. The expression of rat β-defensin-2 mRNA in lung tissue was detected by RT-PCR. The content of β-defensin-2 in alveolar lavage fluid and peripheral blood serum was detected by ELISA. The content of peripheral blood T cell subsets (CD3, CD4, CD8) was detected by flow cytometry, and the ratio of CD4/CD8 was calculated.
RESULTS:After tracheotomy, the levels of β-defensin-2 mRNA and β-defensin-2 in lung tissue from the group B were increased significantly at 24 h, then they were decreased gradually, and decreased most significantly at 168 h (<0.05). The content of β-defensin-2 in peripheral blood of group B decreased gradually, and the content of β-defensin-2 in 168 h was significantly lower than that in 24 h (<0.05), but there was no significant difference between group B and group A (>0.05). The level of CD3 T cells in peripheral blood was significantly lower than that in the group A (<0.05), but their was no significant difference in CD4 and CD8 T cells compared with group A (>0.05). After ICA intervention in group C: lung tissue, alveolar lavage fluid, peripheral blood serum β-defensin-2 content, and peripheral blood CD3 and CD4 T cell levels were gradually increased, significantly higher than those in the group B (<0.05). CD8 T cell level was significantly lower than that in the group A at 24 h (<0.05), the CD4/CD8 ratio was significantly higher at 168 h than those in the group A or B (both <0.01).
CONCLUSIONS:ICA can improve the early lung immune function in rats with tracheotomy, which might be related to up-regulation of β-defensin-2 in lung tissue and alveolar lavage fluid, concomitant with increases in CD3 and CD4 T cells and CD4/CD8 ratio in peripheral blood while reduction in CD8 cells.
