Experience in the extraction and culture of neural stem cells

VernacularTitle:神经干细胞提取及培养方法的体会
Author: Zeng WU ¹
Author Information

1. Department of Pathophysiology, Chengde Medical College, Chengde 067000, China
Publication Type:Journal Article
Keywords: stem cells; neurons; culture media; serial passage; cell extraction; suspension culture method
From: Tianjin Medical Journal 2018;46(8):881-885
CountryChina
Language:Chinese
Abstract: There are many ways to extract and culture neural stem cells in vitro, but the viability and stability of neural stem cells obtained by different methods are different. By thinking about the process of extracting and culturing neural stem cells in vitro from the cerebral cortex of SD fetal rats, we summarized extraction steps, the main points of extraction, the selection basis of culture medium, selection of inoculation density, cultural method, methods of solution changing, passage time and passage methods. At last a large number of neural stem cells with high vitality and stability have been obtained and applied to the basic research of neural stem cells.