Preventive and therapeutic mechanism of ginkgo biloba extract on liver injury poisoned by xylene
- VernacularTitle:银杏叶提取物对二甲苯中毒性肝损伤防治机制的研究
- Author:
Hong-mei MI
1
,
2
Author Information
1. Department of Gastroenterology, The No.4 People&rsquo
2. s Hospital of Hengshui, Hebei 053000, China
- Publication Type:Journal Article
- Keywords:
xylenes;
NF-kappa B;
tumor necrosis factor-alpha;
ginkgo biloba extract;
liver injury
- From:
Tianjin Medical Journal
2018;46(8):847-851
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the potential effect and molecular mechanism of ginkgo biloba extract (GBE) on
the expression of inflammatory factors in liver tissue of mice poisoned by xylene. Methods A total of 30 clean grade
healthy Kunming mice were randomly divided into normal control group, model group and ginkgo biloba extract intervention
group (GBE group). Mice of three groups were granted free access to food and water. The concentration of xylene inhalation
was 110-130 mg/m3 (the air samples were collected in the box, and the gas chromatogram was measured by direct injection
method) in model group and GBE group. The mice in control group inhaled air. GBE group was intraperitoneally injected
with 50 mg/(kg·d) of GBE. Control and model groups were intraperitoneal injected with same amount of normal saline. After
8 weeks, mice were sacrificed, and serum and liver tissues were retained. The serum levels of alanine aminotransferase
(ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), alkaline phosphatase (ALP) and glutamyl transpeptidase
(GGT) were measured. Haematoxylin and Eosin staining was used to observe pathological changes of liver tissue. RT-PCR
and Western blot assay were used to detect the expression levels of nuclear factor kappa B (NF-κB), tumor necrosis factor
alpha (TNF-α) mRNA and protein in liver tissue. Results Compared with the control group, the liver tissue was damage
seriously in the model group. The blood biochemical indexes (ALT, AST, TBIL, ALP and GGT) were significantly increased
(P＜0.05), and the NF-κB, TNF-α mRNA and protein were significantly increased in the model group (P＜0.05). Compared
with the model group, the liver tissue damage was reduced, the blood biochemical indexes (ALT, AST, TBIL, ALP and GGT)
were significantly decreased (P＜0.05) and the NF-κB, TNF-α mRNA and protein were significantly down-regulated in the
GBE group (P＜0.05). Conclusion Ginkgo biloba extract can reduce hepatic inflammatory response of mice poisoned by
xylene through inhibiting the expression of NF-κB and TNF-α inflammatory factors.
