Phylogenetic analysis of Echinococcus granulosus genotypes based on the GenBank database
10.16250/j.32.1374.2019147
- VernacularTitle:基于 GenBank 数据库的不同基因型细粒棘球绦虫系统进化分析
- Author:
Shi-Le WU
1
;
Wen-Jun ZHU
1
,
2
;
De-Yan FAN
3
;
Bing-Qiang SHI
1
;
Yong-De AN
1
;
Chun-Liang WANG
1
;
Xiu-Min HAN
1
;
Ya-Min GUO
1
Author Information
1. Qinghai Provincial People’s Hospital, Qinghai Province, Xining 810007, China
2. Co-first author
3. Qinghai Provincial Red Cross Hospital, China
- Publication Type:Journal Article
- Keywords:
Echinococcus granulosus;
Genotype;
Phylogenetic analysis;
Cytochrome C oxidase subunit I
- From:
Chinese Journal of Schistosomiasis Control
2020;32(1):36-46
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the sequences of the cytochrome C oxidase subunit I (Cox1) gene of various Echinococcus granulosus genotypes that are currently recorded in the GenBank database, so as to investigate the genetic variation and differentiation of the E. granulosus genotypes across the world. Methods The sequences of the Cox1 gene of various E. granulosus genotypes that are currently recorded in the GenBank database were collected, and the same sequences of the Cox1 gene identified from a region were excluded. The mutation sites among the Cox1 gene sequences were identified and a phylogenetic tree was created based on the Cox1 gene. Results Transversion mutation was the predominant type of mutation in the Cox1 gene of E. granulosus. The same Cox1 gene sequence was found in E. granulosus G1, G6 and G7 genotypes isolated from various geographical locations across the world, with the corresponding GenBank accession numbers of KY766891, MH300971 and MH301007, respectively. Phylogenetic analysis revealed that E. granulosus G10 genotype had a remarkable geographical aggregation. Conclusions E. granulosus G1, G6 and G7 genotypes have primitive Cox1 gene sequences. There is a geographical aggregation of the E. granulosus G10 genotype in the phylogenetic tree, which has a tendency towards reproductive isolation.
