Impacts of DES on the expressions of related genes in the gubernaculums testis of newborn mice.
- Author:
Wei-Liao LI
1
;
Xuan ZHANG
2
;
Yuan-Sheng DU
3
;
Jian-Hong LI
3
;
Xue-Wu JIANG
2
Author Information
1. Department of Pediatric Surgery, Foshan Women and Children's Hospital, Southern Medical University, Foshan, Guangdong 528200, China.
2. Department of Pediatric Surgery, Maternity and Child-Care Hospital, Shenzhen University, Shenzhen, Guangdong 518122, China.
3. Department of Pediatric Surgery, The Second Affiliated Hospital of Shantou University School of Medicine, Shantou, Guangdong 515041, China.
- Publication Type:Journal Article
- Keywords:
actin alpha 1;
androgen receptor;
estrogen receptor;
gubernaculum testis;
mouse;
proliferating cell nuclear antigen;
diethylstilbestrol
- MeSH:
Actins;
metabolism;
Animals;
Animals, Newborn;
Cells, Cultured;
Diethylstilbestrol;
pharmacology;
Dimethyl Sulfoxide;
pharmacology;
Estrogen Receptor alpha;
metabolism;
Estrogens, Non-Steroidal;
pharmacology;
Genitalia, Male;
Gubernaculum;
drug effects;
metabolism;
Male;
Mice;
Proliferating Cell Nuclear Antigen;
metabolism;
RNA, Messenger;
metabolism;
Random Allocation;
Receptors, Androgen;
metabolism;
Testis;
drug effects;
metabolism
- From:
National Journal of Andrology
2017;23(7):583-588
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influence of diethylstilbestrol (DES) on the mRNA expressions of the androgen receptor (AR), estrogen receptor α (ERα), proliferating cell nuclear antigen (PCNA), and actin alpha 1 (ACTα1) in the gubernaculums testis of newborn mice and explore their action mechanisms.
METHODS:A total of 140 male Kunming mice were randomly divided into a blank control, a dimethyl sulfoxide (DMSO) control, and 5 experimental groups to be treated subcutaneously with normal saline, DMSO, and DES at 0.02, 0.1, 0.5, 10 and 50 μg per kg of the body weight per day, respectively, at gestation days 9－17. On the first day after birth, the animals were sacrificed and the gubernaculums testis collected for detection of the mRNA expressions of AR, ERα, PCNA and ACTα1 by RT-PCR.
RESULTS:Compared with the DMSO control, the experimental groups, particularly the DES 10 and 50 μg groups, showed significant increases in the mRNA expression of ERα (RE2 = 0.825, P <0.05), but remarkable decreases in those of AR, PCNA and ACTα1 (RA2 = 0.713, RP2 = 0.946, RT2 = 0.960, P <0.01), all in a dose-dependent manner.
CONCLUSIONS:The AR, ERα, PCNA, and ACTα1 mRNA are expressed in the gubernaculum testis of normal newborn mice, and their expression levels may be influenced by intervention with different concentrations of DES during the gestation. Exogenous estrogens may affect the proliferation and contraction of gubernaculum testis cells and consequently the normal development of the testis or even the whole male reproductive system by influencing the metabolism of ER and/or AR.
