Expressions of SLC22A14 and SPAG6 proteins in the ejaculated sperm of idiopathic asthenozoospermia patients.
- Author:
Fang-Yuan HUO
1
;
Yu-Shan LI
1
;
Xi-Yang YANG
1
;
Quan-Xian WANG
1
;
Jun-Jie LIU
1
;
Lin-Kai WANG
1
;
Yan-Hua SU
1
;
Lin SUN
1
Author Information
1. Human Sperm Bank, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.
- Publication Type:Journal Article
- Keywords:
idiopathic asthenozoospermia;
RT-PCR;
Western blot;
solute carrier family 22 member 14;
sperm;
sperm-associated antigen 6
- MeSH:
Asthenozoospermia;
metabolism;
Blotting, Western;
Ejaculation;
Humans;
Male;
Microtubule Proteins;
genetics;
metabolism;
Organic Cation Transport Proteins;
genetics;
metabolism;
Proteomics;
RNA, Messenger;
metabolism;
Sperm Motility;
Spermatozoa;
metabolism
- From:
National Journal of Andrology
2017;23(8):703-707
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expressions of solute carrier family 22 member 14 (SLC22A14) and sperm-associated antigen 6 (SPAG6) in the sperm of idiopathic asthenospermia men.
METHODS:We collected semen samples from 50 idiopathic asthenozoospermia patients and another 50 normal sperm donors, purified the sperm by discontinuous density centrifugation on Percoll gradients, and then determined the mRNA and protein expressions of SLC22A14 and SPAG6 by RT-PCR and Western blot, respectively.
RESULTS:Compared with the normal controls, the idiopathic asthenozoospermia patients showed significantly decreased mRNA expressions of SLC22A14 (0.77 ± 0.08 vs 0.53 ± 0.10, P<0.01) and SPAG6 (0.78 ± 0.09 vs0.52 ± 0.10 , P<0.01) and protein expressions of SLC22A14 (0.80 ± 0.09 vs 0.55 ± 0.10 , P<0.01) and SPAG6 (0.78 ± 0.09 vs 0.56 ± 0.09, P<0.01).
CONCLUSIONS:T The expressions of SLC22A14 and SPAG6 are reduced in the sperm of the patients with idiopathic asthenospermia, which may be one of the important causes of asthenospermia.
