Chinese medicinal compound CFF-1 induces the apoptosis and cycle-arrest of prostate cancer cells via the PI3K/AKT/FOXO1 signaling pathway.
- Author:
Yang ZHANG
1
;
Zhao-Meng WU
2
;
Bo-Han LEI
3
;
Zi-Jie LU
1
;
Qing-Yi ZHU
1
;
Fu-Song XU
4
;
Mao-Sen ZHANG
4
;
Ping LIU
2
Author Information
1. Department of Urology, Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, China.
2. Key Laboratory of Jiangsu Province for Molecular and Medical Biotechnology, School of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu 210023, China.
3. School of Basic Medical Science and Clinical Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 210009, China.
4. Department of Andrology, Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, China.
- Publication Type:Journal Article
- Keywords:
PI3K/AKT/FOXO1 signaling pathway;
apoptosis;
cell cycle;
prostate cancer;
CFF-1
- MeSH:
Antineoplastic Agents, Phytogenic;
pharmacology;
Apoptosis;
drug effects;
Cell Cycle;
drug effects;
Cell Division;
Cell Line, Tumor;
Cell Proliferation;
Cell Survival;
Drugs, Chinese Herbal;
pharmacology;
Forkhead Box Protein O1;
metabolism;
Humans;
Male;
Neoplasm Proteins;
metabolism;
Phosphatidylinositol 3-Kinases;
metabolism;
Phosphorylation;
Prostatic Neoplasms;
drug therapy;
metabolism;
pathology;
Proto-Oncogene Proteins c-akt;
metabolism;
Signal Transduction;
drug effects
- From:
National Journal of Andrology
2017;23(9):828-837
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the apoptosis-inducing effect of the Chinese medicinal compound CFF-1 on prostate cancer cells and its related molecular mechanisms.
METHODS:Normal prostate WPMY-1 cells and prostate cancer LNCaP, CWR22Rv1, PC3 and DU145 cells were treated in dehydrated alcohol with CFF-1 at 0, 2, 5, or 10 mg/ml for 24 hours. Then the viability of the prostate cells was detected by morphological observation, MTT and CCK-8 assay, nuclear condensation and disruption measured by DAPI staining, the cell cycle and apoptosis calculated by flow cytometry, the activity of the PI3K/AKT/FOXO1 signaling pathway and the expressions of its downstream apoptosis- and cycle-related proteins determined by Western blot.
RESULTS:CFF-1 significantly arrested the cell cycle in the G1 phase, decreased the cell viability and increased the nuclear condensation and disruption in a dose-dependent manner, and elevated the apoptosis rate of prostate cancer cells. At the molecular level, CFF-1 dose-dependently reduced the activity of the PI3K/AKT signaling pathway and phosphorylation of the FOXO1 protein, increased the transcription activity of FOXO1, and eventually regulated the expressions of cell apoptosis- and cycle-related genes.
CONCLUSIONS:The Chinese medicinal compound CFF-1 can significantly inhibit the growth, arrest the cycle, and induce the apoptosis of prostate cancer cells by decreasing the activity of the PI3K/AKT/FOXO1 signaling pathway, which suggests its potential clinical application value in the treatment of prostate cancer.
