Yam polysaccharide improves sperm viability and protects sperm DNA integrity in vitro.
- Author:
Mei-Hua ZHANG
1
;
Zhen-Ya FANG
1
;
An-Na LI
1
;
Yan-Ping ZHANG
1
;
Yi QIU
1
Author Information
1. Center of Genetic Medicine / Key Laboratory for Birth Control Technology of the Health and Family Planning Commission of China, Maternity and Child Health Hospital of Shandong Province, Jinan, Shandong 250014, China.
- Publication Type:Journal Article
- Keywords:
in vitro;
oligoasthenozoospermia;
sperm DNA integrity;
sperm viability;
yam polysaccharide
- MeSH:
Adult;
Ascorbic Acid;
pharmacology;
DNA;
drug effects;
DNA Fragmentation;
Dioscorea;
chemistry;
Humans;
Male;
Polysaccharides;
pharmacology;
Semen Analysis;
Sperm Motility;
Spermatozoa;
drug effects;
physiology;
Vitamins;
pharmacology
- From:
National Journal of Andrology
2017;23(11):1020-1024
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To clarify the roles of yam polysaccharide (YPS) in improving sperm viability and protecting sperm DNA integrity in vitro and provide a new approach to the treatment of oligoasthenozoospermia.
METHODS:We collected samples by masturbation from 36 normal fertile males aged 27－39 years. Each sample was divided into six groups: blank control or treated with normal saline, vitamin C solution, and YPS solution at low (0.25 mg/ml), medium (1.0 mg/ml) or high concentration (5.0 mg/ml). Using eosin-Y staining, sperm hypotonic swelling (HOS) and sperm chromatin diffusion (SCD) test, we observed the effects of different concentrations of YPS on sperm viability, membrane integrity and nuclear DNA.
RESULTS:After 24 and 48 hours of treatment, sperm viability was markedly reduced in the vitamin C (［28.5 ± 3.1］ and ［6.5 ± 1.2］%), low-YPS (［31.3 ± 3.5］ and ［6.5 ± 2.2］%), medium-YPS (［37.1 ± 3.5］ and ［9.5 ± 2.8］%) and high-YPS groups (［38.3 ± 3.3］ and ［9.0 ± 3.2］%) as compared with the blank control (［17.3 ± 2.1］ and ［3.2 ± 1.3］%) (P <0.01) and normal saline groups (［13.4 ± 4.1］ and ［3.1 ± 2.0］%) (P <0.01), and it was significantly higher in the medium- and high-YPS than in the vitamin C group (P <0.05 and P <0.01). The rate of sperm DNA fragmentation was remarkably decreased at 48 hours in the vitamin C (［30.5 ± 3.1］%), low-YPS (［29.4 ± 2.6］%), medium-YPS (［28.5 ± 2.3］%) and high-YPS groups (［27.9 ± 1.9］%) in comparison with the blank control (［41.7 ± 2.2］%) (P <0.01) and normal saline groups (［42.1 ± 3.3］%), markedly lower in the medium- and high-YPS than in the blank control, normal saline and vitamin C groups (P <0.05 or P <0.01), but with no statistically significant difference between the low-YPS and vitamin C groups (P >0.05).
CONCLUSIONS:Yam polysaccharide can improve sperm viability and protect sperm DNA integrity in vitro.
