Application value of frozen-thawed mouse round spermatids in in vitro fertilization.
- Author:
Hong JIANG
1
;
Li WANG
2
;
Cun-Li WANG
1
;
Wen-Xiang ZHANG
1
;
Feng NI
1
Author Information
1. Center of Reproductive Medicine, The 105th Hospital of PLA, Hefei, Anhui 230031, China.
2. Department of Gynecology and Obstetrics, The Second People's Hospital of Anhui Province, Hefei, Anhui 230031, China.
- Publication Type:Journal Article
- Keywords:
freezing-thawing； mouse;
in vitro fertilization;
round spermatid
- MeSH:
Animals;
Cryopreservation;
Embryonic Development;
Female;
Fertilization in Vitro;
Male;
Mice;
Oocytes;
Pregnancy;
Sperm Injections, Intracytoplasmic;
Spermatids;
transplantation
- From:
National Journal of Andrology
2017;23(12):1059-1062
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the application value of the frozen-thawed round spermatids of the mouse in in vitro fertilization (IVF).
METHODS:Haploid spermatids of the mouse obtained in vitro were divided into a frozen-thawed and a fresh group and oocytes were collected from 6－8 weeks old female mice. After diamidino-phenyl-indole (DAPI) staining, the oocytes were subjected to intracytoplasmic round spermatid injection (ROSI), 259 in the frozen-thawed and 238 in the fresh group. Comparisons were made between the two groups in the capacities of fertilization and embryonic development.
RESULTS:The survival rate of the frozen-thawed haploid spermatids was (75.9 ± 2.3) %. No statistically significant differences were observed between the frozen-thawed and fresh groups after ROSI in the rates of fertilization (51.9 vs 55.7%, P >0.05), 2-cell embryos (51.0 vs 62.2%, P >0.05), 4－8-cell embryos (41.8 vs 42.9%, P >0.05), or morula-blastocysts (12.2 vs 21.4%, P >0.05).
CONCLUSIONS:Frozen-thawed round spermatids of the mouse are similar to fresh ones in their capacities of fertilization and embryonic development.
