Huangjing Zanyu Capsule enhances sperm mitochondrial membrane potential in asthenozoospermia patients.
- Author:
Jian-Lin HU
1
;
Jian SUN
2
;
Wei CHEN
2
;
Yi-Xia YANG
2
;
Yuan XU
2
;
Yu WU
2
;
Xiao-Wen SUN
1
;
Zheng LI
2
Author Information
1. Shanghai Yiyuan School of Clinical Medicine, Nanjing Medical University, Shanghai 201620, China.
2. Department of Reproductive Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, China.
- Publication Type:Journal Article
- Keywords:
asthenozoospermia;
mitochondrial membrane potential;
Huangjing Zanyu Capsule
- MeSH:
Asthenozoospermia;
drug therapy;
Capsules;
Case-Control Studies;
Drugs, Chinese Herbal;
administration & dosage;
therapeutic use;
Female;
Flow Cytometry;
Humans;
Male;
Membrane Potential, Mitochondrial;
drug effects;
physiology;
Pregnancy;
Semen;
drug effects;
Sperm Motility;
drug effects;
Spermatozoa;
drug effects;
ultrastructure;
Staining and Labeling
- From:
National Journal of Andrology
2017;23(12):1116-1120
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of Huangjing Zanyu Capsule (HZC) on sperm mitochondrial membrane potential (MMP) in asthenozoospermia patients.
METHODS:We assigned 70 asthenozoospermia patients to a treatment group (n = 39) and a control group (n = 31), the former treated with oral HZC at the dose of 4 capsules tid for 3 months while the latter left untreated. We obtained semen parameters from the patients and detected their sperm mitochondrial membrane potentials (MMP) by JC-1 staining and flow cytometry before and after medication, followed by comparison between the two groups.
RESULTS:The total effectiveness rate was 71.05% in the treatment group and natural pregnancy was achieved in 3 cases during the medication. A total of 35 patients in the treatment group and 30 controls completed all the laboratory examinations after a 3-month observation. Compared with the controls, the patients treated with HZC exhibited significant improvement after medication in MMP (variation value: ［1.19 ± 10.36］% vs ［20.28 ± 14.21］%, P <0.01), total sperm motility (variation value: ［3.46 ± 8.67］% vs ［20.68 ± 14.12］%, P <0.01), the percentage of progressively motile sperm (variation value: ［2.26 ± 8.29］% vs ［17.58 ± 12.73］%, P <0.01), and the percentage of morphologically normal sperm (variation value: ［0.23 ± 3.48］% vs ［3.37 ± 3.99］%, P <0.01). MMP was significantly correlated with total sperm motility (r = 0.69, P <0.01), progressive sperm motility (r = 0.75, P <0.01) and normal sperm morphology (r = 0.26, P <0.01).
CONCLUSIONS:Huangjing Zanyu Capsule can enhance sperm mitochondrial membrane potential and sperm mitochondrial function, thus improving total sperm motility, progressive sperm motility and normal sperm morphology. It is safe and effective for the treatment of asthenospermia.
