Expression characteristics of the Daxx gene in the mouse testis during spermatogenesis.
- Author:
Zeng ZHANG
1
;
Qiong DENG
2
;
Yong WU
2
;
Xin-Bo HUANG
2
;
Lu YAO
2
;
Zhi-Mao JIANG
2
;
Yao-Ting GUI
1
Author Information
1. Graduate School, Anhui Medical University, Hefei, Anhui 230032, China.
2. Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, China.
- Publication Type:Journal Article
- Keywords:
death domain associated protein (Dxxx) gene;
mouse;
spermatogenesis;
testis
- MeSH:
Animals;
Carrier Proteins;
genetics;
metabolism;
Cell Nucleus;
genetics;
metabolism;
Gene Expression;
Intracellular Signaling Peptides and Proteins;
genetics;
metabolism;
Male;
Mice;
Mice, Knockout;
Molecular Chaperones;
Nuclear Proteins;
genetics;
metabolism;
Receptors, Androgen;
genetics;
Sertoli Cells;
Spermatogenesis;
genetics;
Testis;
metabolism
- From:
National Journal of Andrology
2017;23(2):103-109
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression characteristic of the Daxx gene in the mouse testis and its role in spermatogenesis.
METHODS:Real-time PCR, Western blot and immunofluorescence were used in examining the expression characteristics of DAXX in the testis tissue from wild-type, Sertoli cell-specific androgen receptor knockout (SCARKO) and androgen receptor knockout (ARKO) mice at different postnatal weeks .
RESULTS:The Daxx gene was highly expressed in the testis tissue and mainly in the nuclei of the wild-type mice at 4 postnatal weeks. Compared with the wild-type, the ARKO mice showed a markedly decreased expression of DAXX (0.299±0.026), which displayed a polar distribution in the spermatogenic cells (0.853±0.058) and exhibited no significant difference in the SCARKO mice (1.000±0.015).
CONCLUSIONS:The Daxx gene expression is the highest in the middle-stage development of the mouse testis, significantly decreased in ARKO mice as compared with the wild-type, and its location influenced by specific AR knockout in Sertoli cells. DAXX may be involved in the regulation of spermatogenesis in mice.
