Expression of long non-coding RNA H19 in prostate cancer and its effect on the proliferation and glycometabolism of human prostate cancer cells.
- Author:
Shi-Cheng SUN
1
;
Hu ZHAO
1
;
Rui LIU
1
;
Bi-Liang WANG
1
;
Yi-Qing LIU
1
;
Yan ZHAO
2
;
Zhen-Duo SHI
2
Author Information
1. Department of Urology, Guanyun People's Hospital, Lianyungang, Jiangsu 222200, China.
2. Department of Urology, Xuzhou Central Hospital, Southeast University, Xuzhou, Jiangsu 210000, China.
- Publication Type:Journal Article
- Keywords:
DU145;
H19;
PC-3;
glycometabolism;
long non-coding RNA;
proliferation;
prostate cancer
- MeSH:
Cell Line, Tumor;
Cell Proliferation;
Gene Expression Regulation, Neoplastic;
Glucose;
metabolism;
Humans;
Lactic Acid;
metabolism;
Male;
Prostate;
metabolism;
Prostatic Hyperplasia;
metabolism;
Prostatic Neoplasms;
metabolism;
pathology;
RNA, Long Noncoding;
genetics;
metabolism;
RNA, Small Interfering;
Transfection
- From:
National Journal of Andrology
2017;23(2):120-124
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression of long noncoding RNA (lncRNA) H19 in human prostate cancer tissue and its effect on the glycometabolism and growth of human prostate cancer cells.
METHODS:Realtime quantitative RTPCR (qRTPCR) was employed to detect the expression of lncRNA H19 in human prostate tissues from 20 patients with prostate cancer (10 cases of highGleason score prostate cancer ［HGPC］ and 10 cases of lowGleason score prostate cancer ［LGPC］) and another 5 with benign prostatic hyperplasia (BPH). After transfection of H19 siRNA into the DU145 and PC3 prostate cancer cells, the growth of the cells and the H19 expression in the cells were determined by MTT and qRTPCR respectively, and the changes in the glycometabolism of the prostate cancer cells were analyzed by measuring the contents of glucose and lactate in the culture medium. Nontransfected and transfected negative vectors were used as blank and negative controls respectively.
RESULTS:The relative expression of H19 was significantly increased in both the HGPC and LGPC tissues (0.725±0.385 and 2.086±0.542) as compared with that in the BPH tissue (0.210±0.068) (P< 0.01), even higher in the HGPC than in the LGPC tissue (P< 0.01). After transfection of H19 siRNA, the expressions of H19 were remarkably decreased in the DU145 and PC3 prostate cancer cells in comparison with those in the blank control and negative control groups (P< 0.01), and so were the proliferation of and the glucose and lactate levels in the DU145 and PC3 cells (P< 0.01).
