Combination of musk and olibanum promotes proliferation of prostate stem cells in mice: An experimental study.
- Author:
Qun-Fang LIN
1
;
Pei HUANG
1
;
Xue-Jun SHANG
2
;
Xue-Fei TIAN
3
;
Guo-Min ZHANG
3
;
Rui-Song GAO
4
;
Xing ZHOU
4
;
Hui-Ying LIU
1
;
Qing ZHOU
4
Author Information
1. Graduate School, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China.
2. Department of Andrology, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu,210002, China.
3. School of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China.
4. Department of Andrology, The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China.
- Publication Type:Journal Article
- Keywords:
musk;
mice;
olibanum;
prostate stem cell;
urogenital sinus mesenchymal cell
- MeSH:
Animals;
Cell Differentiation;
drug effects;
Cell Proliferation;
drug effects;
Drug Therapy, Combination;
Epithelial Cells;
cytology;
drug effects;
Fatty Acids, Monounsaturated;
pharmacology;
Female;
Frankincense;
pharmacology;
Male;
Mesenchymal Stem Cells;
cytology;
drug effects;
Mice;
Mice, Inbred C57BL;
Mice, SCID;
Pregnancy;
Prostate;
cytology;
Random Allocation;
Receptor Protein-Tyrosine Kinases;
Receptors, Cholinergic;
Stem Cells;
cytology;
drug effects
- From:
National Journal of Andrology
2017;23(2):157-163
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of muskolibanum combination on the proliferation and differentiation of prostate stem cells.
METHODS:We cultured prostate epithelial cells and urogenital sinus mesenchymal (UGSM) cells from 7－10 d old C57BL/6 mice and 16－18 d old pregnant C57BL/6 mice, transplanted the mixed suspension of the two types of cells under the kidney envelope of SCIDCB.17 male mice, and harvested the transplants 30 days later. We randomly divided the SCIDCB.17 mice into four groups to be treated intragastrically with musk (n = 8), olibanum (n = 8), musk+olibanum (n = 7), and normal saline (blank control, n = 8)) respectively, all for 14 days. Then we collected the kidney tissue for observation of the morphology of the glandular tubes and differentiation of different subsets of stem cells by HE staining and determination of the expressions and distribution of P63, CD133, CD117 and Sca1 by immunohistochemistry and Western blot.
RESULTS:A system was successfully established for the isolation and mixed culture of Sca1 Lin+ CD49f+ (LSC) cells of prostate stem cells and UGSM cells of the mouse embryonic prostate. Immunohistochemistry showed positive expressions of P63, CD133, Sca1, and CD117 in the prostatic acinar epithelia and proved the presence of prostatic acinar epithelial structure in the transplants. Compared with the blank control group, the expressions of CD133, Sca1 and CD117 were significantly increased in the musk, olibanum, and musk+olibanum groups (P< 0.05), higher in the musk+olibanum than in the musk or olibanum group (P< 0.05), and their protein expressions were even more elevated in the musk+olibanum group (P< 0.01), with statistically significant difference from the olibanum group (P< 0.05).
CONCLUSIONS:The combination of musk and olibanum can improve the proliferation and differentiation of prostate stem cells.
