Protective effect of astaxanthin against epididymal oxidative damagein rats with ornidazole-induced oligoasthenozoospermia.
- Author:
Wei LIU
1
;
Xiao-Fang KANG
2
;
Guo-Wei ZHANG
1
;
Hong-Cai CAI
1
;
Kai-Qiang LI
1
;
Ling-Ling WANG
3
;
Xue-Jun SHANG
1
Author Information
1. Department of Andrology, Nanjing School of Clinical Medicine, Southern Medical University/ Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China.
2. Department of Hematology,The First School of Clinical Medicine, Shanxi Medical University, Taiyuan, Shanxi 030001, China.
3. Department of Medical Affairs, Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China.
- Publication Type:Journal Article
- Keywords:
SD rat;
antioxidant;
astaxanthin;
oligoasthenozoospermia;
ornidazole;
oxidative damage
- MeSH:
Animals;
Antioxidants;
pharmacology;
Asthenozoospermia;
prevention & control;
Epididymis;
drug effects;
metabolism;
Male;
Oligospermia;
prevention & control;
Ornidazole;
Oxidative Stress;
Protective Agents;
pharmacology;
Radiation-Sensitizing Agents;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Sperm Count;
Sperm Motility;
Spermatozoa;
drug effects;
metabolism;
Xanthophylls;
pharmacology
- From:
National Journal of Andrology
2017;23(3):206-211
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the improving effect of astaxanthin (AST) on the sperm quality of rats with ornidazole (ORN)-induced oligoasthenozoospermiaand its action mechanism.
METHODS:Forty adult male SD rats were equally randomized into groups A (solvent control), B (low-dose ORN ［400 mg/（kg·d）］), C (high-dose ORN ［800 mg/（kg·d）］), D (low-dose ORN ［400 mg/（kg·d）］ + AST ［20 mg/（kg·d）］), and E (high-dose ORN ［800 mg/（kg·d）］ + AST ［20 mg/（kg·d）］), all treated intragastrically for3 weeks.After treatment, the epididymal tails ononeside was taken for determination of sperm concentration and activity, and the epididymideson the other side harvested for measurement of the activities of GSH-Px, GR, CAT and SOD and the MDA contentin the homogenate.
RESULTS:Compared with group A, sperm motilityin the epididymal tail andGSH-Px and SOD activities in theepididymiswere markedly decreased while the MDAcontent significantlyincreased in group B (P<0.05), spermmotility and concentrationin the epididymal tail, testisindex, and the activities of GSH-Px, GR, CAT and SOD in the epididymis were remarkably reduced while theMDA contentsignificantly increased in group C(P<0.05). In comparison with group B, group D showed markedly increased sperm motility (［45.3±8.7］% vs ［66.3±8.9］%, P<0.05) in the epididymal tail and SOD activity in the epididymis (［116.7±25.3］ U/mg prot vs ［146.1±23.8］ U/mg prot, P<0.05), decreased MDA content(［1.68±0.45］ nmol/mg prot vs ［1.19±0.42］ nmol/mg prot, P<0.05).Compared with group C, group Eexhibited significant increases in the weight gained (［89.0±9.5］ vs ［99.9±4.1］ %, P<0.05) and sperm motility (［17.9±3.5］% vs ［27.3±5.3］ %, P<0.05) but a decrease in the content of MDA (［2.03±0.30］ nmol/mg prot vs ［1.52±0.41］ nmol/mg prot, P<0.05).
CONCLUSIONS:AST can improve spermquality in rats with ORN-inducedoligoasthenozoospermia, which may be associated with its enhancing effect on the antioxidant capacity of the epididymis.
