Expressions of TRPV1 and TRPA1 in the dorsal root ganglion in the rat model of orchialgia.
- Author:
Jing-Wei YU
1
;
Jie-Hong HUANG
2
;
Kun-Long LÜ
1
;
Ming-Kuan ZHOU
1
;
Xin FENG
1
;
Kun TIAN
1
;
Jin-Tao ZHUANG
1
;
Wen-Liang ZHOU
2
;
Chun-Hua DENG
1
;
Xiang-An TU
1
Author Information
1. Department of Urology and Andrology, The First Hospital Affiliated to Sun Yat-sen University, Guangzhou, Guangdong 510080, China.
2. School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510275, China.
- Publication Type:Journal Article
- Keywords:
chronic orchialgia;
dorsal root ganglion;
rat;
transient receptor potential ankyrin 1;
transient receptor potential vanilloid 1
- MeSH:
Acetic Acid;
Animals;
Ganglia, Spinal;
metabolism;
Hyperalgesia;
chemically induced;
metabolism;
Male;
Membrane Glycoproteins;
Oxidoreductases;
Rats;
Rats, Sprague-Dawley;
TRPA1 Cation Channel;
metabolism;
TRPV Cation Channels;
metabolism;
Testicular Diseases;
chemically induced;
metabolism;
Up-Regulation
- From:
National Journal of Andrology
2017;23(4):296-301
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expressions of transient receptor potential vanilloid 1 (TRPV1) and TRP ankyrin 1 (TRPA1) in the dorsal root ganglion (DRG) and their action mechanisms in the rat model of orchialgia.
METHODS:The models of orchialgia were established in male SD rats by injection of 2% acetic acid into the testis. Then the number of spontaneous pain responses and withdrawal latency in the model rats were recorded by behavioral tests and the expressions of TRPV1 and TRPA1 in T13－L1 DRGs determined by RT-qPCR, Western blot and immunofluorescence staining.
RESULTS:Compared with the normal control rats, the orchialgia models showed a significant increase in the number of spontaneous pain responses (0.13 ± 0.35 vs 22.63 ± 3.42, P<0.01) and a decrease in the withdrawal latency at 4 hours after injection (［12.75 ± 1.50］ vs ［4.85 ± 1.00］ s, P<0.05). The mRNA expressions of both TRPV1 and TRPA1 were observed in the membrane of the neurons in the DRG, the former increased by 1.77 times and the latter by 1.75 times that of the control (P<0.05).
CONCLUSIONS:The expressions of TRPV1 and TRPA1 were up-regulated in the DRG of the rat models of orchialgia, which may be involved in the allodynia and hyperalgesia of the rats.
