Sperm chromatin structure assay versus sperm chromatin dispersion test in detecting sperm DNA integrity and correlation of sperm DNA fragmentation with semen parameters.
- Author:
Jia-Xiong WANG
1
;
Mu-Tian HAN
1
;
Li-Yan SHEN
1
;
Gai-Gai WANG
1
;
Hong-Bo CHENG
1
;
Shen-Min YANG
1
;
Dan SONG
1
;
Wei WANG
1
;
Hong LI
1
;
Yi-Chao SHI
1
Author Information
1. Center of Reproduction and Genetics, Suzhou Municipal Hospital Affiliated to Nanjing Medical University, Suzhou, Jiangsu 215002, China.
- Publication Type:Journal Article
- Keywords:
sperm chromatin structure assay;
age;
sperm DNA fragmentation;
sperm chromatin dispersion test;
sperm motility;
percentage of morphologically normal sperm
- MeSH:
Chromatin;
genetics;
physiology;
DNA Fragmentation;
Humans;
Infertility, Male;
diagnosis;
Male;
Semen;
physiology;
Semen Analysis;
Sperm Motility;
Spermatozoa;
physiology;
ultrastructure
- From:
National Journal of Andrology
2017;23(4):329-336
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Sperm DNA fragmentation (SDF) is widely used to predict male infertility and the methods of detecting SDF are varied. This study aimed to compare two methods of SDF detection and investigate the correlation between SDF and sperm quality.
METHODS:Using sperm chromatin structure assay (SCSA) and sperm chromatin dispersion test (SCD), we detected SDF in 108 semen samples collected in the Center of Reproduction and Genetics of Suzhou Municipal Hospital. We compared the results of the two methods and analyzed the correlations of SDF routine semen parameters, sperm morphology and the age of the patients.
RESULTS:A significant consistency was found in the SDF index (DFI) between the two methods (P<0.01). The DFI was correlated negatively with sperm motility, the percentage of progressively motile sperm, and that of morphologically normal sperm (P <0.01), but positively with the teratozoospermia index (P <0.01 in SCSA and P <0.05 in SCD). The DFI measured by SCSA showed a significantly positive correlation with the patients' age (P <0.01), but not that obtained by SCD.
CONCLUSIONS:The results of both SCSA and SCD play an important role in predicting sperm quality. As a clinical index, the DFI has a predictive value for male infertility. However, the results of different detecting methods vary widely, which calls for further studies on their standardization.
