Effects of obesity on global genome DNA methylation and gene imprinting in mouse spermatozoa.
- Author:
Jin-Liang ZHU
1
;
Yin-Ling WU
2
;
Wen-Hao TANG
3
;
Yuan TIAN
2
;
Shao-Qin GE
2
;
Ping LIU
1
;
Jie QIAO
1
Author Information
1. Center of Reproductive Medicine, Department of Gynecology and Obstetrics, Peking University Third Hospital, Beijing 100191, China.
2. Hebei University School of Medicine, Baoding, Hebei 071000, China.
3. Department of Urology, Peking University Third Hospital, Beijing 100191, China.
- Publication Type:Journal Article
- Keywords:
gene imprinting;
global genome methylation;
sperm;
obesity
- MeSH:
Animals;
DNA Methylation;
Diet, High-Fat;
Genome;
Genomic Imprinting;
Insulin-Like Growth Factor II;
Male;
Mice;
Obesity;
genetics;
metabolism;
RNA;
biosynthesis;
Spermatozoa;
metabolism
- From:
National Journal of Andrology
2017;23(6):488-496
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influence of high fat diet-induced obesity (HFDIO) on the differentially methylated region (DMR) of the imprinted gene and global genome methylation of sperm DNA.
METHODS:We performed bisulfite sequencing on the DMR of the imprinted gene and global genome methylation of sperm DNA in the mouse model of HFDIO.
RESULTS:No statistically significant differences were found between the HFDIO model and normal control mice in MEG3-IG (93.73 vs 97.26%, P = 0.252), H19 (98.00 vs 97.83%, P = 0.920), IGF2 (97.34 vs 96.25%, P =0.166), IGF2R (1.43 vs 1.11%, P = 0.695), PEG3 (0.19 vs 0.38%, P = 0.537), MEST (0.23 vs 0.68%, P = 0.315), NNAT (0.31 vs 0.00%, P = 0.134), or SNRPN (1.88 vs 3.13%, P = 0.628). A total of 8 942 DMRs were detected across the sperm genome (P <0.05). Gene functional enrichment analysis indicated that the enriched terms with the largest numbers of genes were the metabolic process (n = 1 482), RNA synthesis (n = 779), and transcription (n = 767).
CONCLUSIONS:The methylation level underwent no significant change in the DMRs of the imprinted genes from the mice with HFDIO, but the CG methylation of the genes involved in the metabolic process, RNA synthesis and transcription were significantly altered.
