Role of TGF-β1 in Sertoli cells and tight junction.
- Author:
Jiang-Tao LOU
1
;
Ren-Xiong WEI
1
;
Lang-Lang YU
1
;
Jian-Wei CHEN
1
;
Yun CUI
1
Author Information
1. Laboratory of Andrology, Ningbo Hospital of Traditional Chinese Medicine, Zhejiang University of Chinese Medicine, Ningbo, Zhejiang 315012, China.
- Publication Type:Journal Article
- Keywords:
Occludin;
Sertoli cell;
rat;
tight junction;
TGF-β1
- MeSH:
Animals;
Apoptosis;
Cell Proliferation;
Cells, Cultured;
Claudin-2;
metabolism;
Male;
Occludin;
metabolism;
RNA, Messenger;
Rats;
Sertoli Cells;
cytology;
physiology;
Tight Junction Proteins;
metabolism;
Tight Junctions;
genetics;
metabolism;
Transforming Growth Factor beta1;
physiology;
Zonula Occludens-1 Protein;
metabolism
- From:
National Journal of Andrology
2017;23(6):510-516
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role of TGF-β1 in the proliferation and apoptosis of Sertoli cells and its effect on the expressions of tight junction-related proteins and genes in rats.
METHODS:Rat Sertoli cells were isolated in vitro, primarily cultured, and divided into groups A (blank control), B (TGF-β1 receptor blocker), C (TGF-β1), and D (TGF-β1 + receptor blocker). The proliferation and apoptosis of the cells were detected by CCK-8 and flow cytometry, respectively. After establishment of the dual-chamber model for the primary culture of Sertoli cells, the trans-epithelia electrical resistance (TER) value was measured and the relative expressions of Occludin, ZO-1 and Claudin Ⅱ determined by RT-PCR and Western blot.
RESULTS:The OD value of the proliferation of the Sertoli cells was markedly higher in group C than in groups A and D (0.79 ± 0.04 vs 0.66 ± 0.05 and 0.68 ± 0.02, P<0.05), with statistically significant differences among the four groups (F = 5.05, P <0.05). However, no remarkable difference with found among the four groups in the apoptosis rate of the cells (F = 1.13, P >0.05). The TER value was dramatically decreased in group C as compared with groups A and D (［176.37 ± 16.61］ vs ［281.42 ± 9.83］ and ［254.37 ± 13.55］ /cm2, P<0.01), with statistically significant differences among the four groups (F = 38.99, P<0.01). There were no remarkable differences among the four groups in the mRNA expressions of ZO-1 and Claudin Ⅱ (F = 0.49 and 0.93, P>0.05) or their protein expressions (F = 0.28 and 1.31, P>0.05). Both the mRNA and protein expressions of Occludin were markedly lower in group C than in A and D (P<0.01 and P<0.05), with statistically significant differences among the four groups (F = 6.86 and 6.87, P<0.01).
CONCLUSIONS:TGF-β1 can promote the proliferation of Sertoli cells in rats and act on the tight junction of the cells by regulating the expression of Occludin.
