Anti-fertility effect of flower extracts of Tabernaemontana divaricata in rats.
10.1016/S1875-5364(12)60013-2
- Author:
Mohd Azeemuddin MUKHRAM
1
;
H SHIVAKUMAR
;
G L VISWANATHA
;
S RAJESH
Author Information
1. Department of Pharmacology, SCS College of Pharmacy, Harapanahalli, Davanagere (District), Karnataka, India.
- Publication Type:Journal Article
- MeSH:
Abortifacient Agents;
pharmacology;
Animals;
Embryo Implantation;
drug effects;
Female;
Fertility;
Flowers;
chemistry;
Humans;
Models, Animal;
Organ Size;
drug effects;
Phytoestrogens;
pharmacology;
Plant Extracts;
chemistry;
pharmacology;
Rats;
Rats, Wistar;
Tabernaemontana;
chemistry;
Uterus;
drug effects
- From:
Chinese Journal of Natural Medicines (English Ed.)
2012;10(1):58-62
- CountryChina
- Language:English
-
Abstract:
AIM:To evaluate the anti-fertility effect of methanolic (MeTD) and aqueous (AqTD) flower extracts of Tabernaemontana divaricata in rats.
METHODS:The anti-fertility activity of the extracts was evaluated using two experimental animal models: 1) Estrogenic activity was carried out in immature female rats using ethinyl estradiol as standard. The evaluation parameters includes changes in uterine weight and histopathology of uterus. 2) Anti-implantation and early abortifacient activity was performed in female Wistar rats. The number of implants and resorbtions were compared to vehicle control.
RESULTS:Phytochemical analysis of MeTD and AqTD revealed the presence of carbohydrates, amino acids, steroids, glycosides, flavonoids, alkaloids and tannins. In estrogenic activity, the MeTD and AqTD were offered significant estrogen-like activity at 500 mg·kg(-1), p.o. by increasing the uterine weight compared to vehicle control group. In Anti-implantation and early abortifacient activity study, MeTD (500 mg·kg(-1), p.o.) showed significant effect and it was evident by decrease in the number of implants and increase in the number of resorbtions compared to vehicle control group.
CONCLUSION:The MeTD at 500 mg·kg(-1), p.o. possess significant estrogenic, anti-implantation and early abortifacient activity, while the AqTD at 500 mg·kg(-1), p.o. was found to possess significant estrogenic activity and the results are in consistent with the literature reports related to anti-fertility effect of flower extracts of Tabernaemontana divaricata.
