Protective effects of shengmai san and its three fractions on cerebral ischemia-reperfusion injury.
10.1016/S1875-5364(13)60020-5
- Author:
Lai-Hong LI
1
;
Jun-Song WANG
;
Ling-Yi KONG
Author Information
1. State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Chromatography, High Pressure Liquid;
Disease Models, Animal;
Drugs, Chinese Herbal;
administration & dosage;
chemistry;
Glutathione Peroxidase;
genetics;
metabolism;
Humans;
Male;
Malondialdehyde;
metabolism;
Nitric Acid;
metabolism;
Oxidative Stress;
drug effects;
Peroxidase;
genetics;
metabolism;
Protective Agents;
administration & dosage;
chemistry;
Rats;
Reperfusion Injury;
drug therapy;
genetics;
metabolism;
prevention & control;
Superoxide Dismutase;
genetics;
metabolism
- From:
Chinese Journal of Natural Medicines (English Ed.)
2013;11(3):222-230
- CountryChina
- Language:English
-
Abstract:
AIM:To investigate the antioxidant and anti-inflammatory effects of Shengmai San (SMS) and its ethyl acetate extract (SEa), n-butanol extract (SBu), and aqueous extract (SWe), and clarify the material base of SMS and the roles played by its fractions.
METHODS:A mouse model of transient forebrain ischemia/reperfusion (I/R) by means of common carotid artery occlusion (CCAO) was used to investigate the effects of SMS and its three fractions. Histopathological damage, blood-brain barrier disruption, and antioxidant and inflammation-related parameters, including malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx), myeloperoxidase (MPO), nitric oxide (NO), tumor necrosis factor-α (TNF-α) were measured. The chemical constituents of each fraction were identified by LC-MS.
RESULTS:Eighteen lignans in SEa, and thirteen steroidal glycosides and ginsenosides in SBu were determined. SMS significantly inhibited I/R induced formation of histological injury and cerebral MPO activity. SMS showed the strongest antioxidant and anti-inflammatory effects against the I/R-caused injuries. SEa showed higher antioxidant activity than the other two fractions and SBu has a slightly stronger inhibition on the productions of NO and TNF-α.
CONCLUSION:SMS as a whole had the most effective protection against cerebral I/R-caused injuries compared with its fractions, which inferred that it contains different groups of compounds that contribute together to its protective effect.
