Effects of salvianolic acid and notoginseng triterpenes on angiogenesis in EA-hy926 cells in vitro.
10.1016/S1875-5364(13)60024-2
- Author:
Hai-Bo SHI
1
;
Jian-Dong CHEN
;
Xiao-Hu CHEN
;
Yun HE
;
Zhi-Jian YANG
Author Information
1. Nanjing Medical University, Nanjing, China.
- Publication Type:Journal Article
- MeSH:
Alkenes;
pharmacology;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Coronary Stenosis;
drug therapy;
genetics;
metabolism;
physiopathology;
Drugs, Chinese Herbal;
pharmacology;
Endothelial Cells;
drug effects;
metabolism;
Humans;
Neovascularization, Pathologic;
drug therapy;
genetics;
metabolism;
physiopathology;
Panax notoginseng;
chemistry;
Polyphenols;
pharmacology;
Triterpenes;
pharmacology;
Vascular Endothelial Growth Factor A;
genetics;
metabolism
- From:
Chinese Journal of Natural Medicines (English Ed.)
2013;11(3):254-257
- CountryChina
- Language:English
-
Abstract:
AIM:To investigate the different effects of salvianolic acid and notoginseng triterpenes on proliferation, angiogenesis and expression of vascular endothelial growth factor in EA-hy926 cells in vitro.
METHODS:EA-hy926 cells were cultured in vitro. Salvianolic acid and notoginseng triterpenes at concentrations of 0.4, 0.8 and 1.2 mg·L(-1) were used to culture EA-hy926 cells. EA-hy926 cells in a blank control group were grown in culture solution only. Viability of cells was assessed by CCK-8, and after treated for 12 h, capillary-like structures were examined. After 24 h culture, the expression of VEGF was detected by real-time PCR.
RESULTS:Salvianolic acid at 0.4, 0.8 mg·L(-1), the same as notoginseng triterpenes, increased VEGF content in EA-hy926 cells. Expression of VEGF protein in the salvianolic acid at 1.2 mg·L(-1) group, was up-regulated as compared with notoginseng triterpenes group (P < 0.05).
CONCLUSION:Salvianolic acid and notoginseng triterpenes can promote EA-hy926 cell proliferation, angiogenesis and expression of VEGF protein. This analysis also provided evidence that salvianolic acid had the better effects as compared with notoginseng triterpenes.
