Radioprotective effect of adenine on irradiation-induced apoptosis.
10.1016/S1875-5364(13)60040-0
- Author:
Xiao-Yan WANG
1
;
Zeng-Chun MA
;
Shuai SHAO
;
Qian HONG
;
Yu-Guang WANG
;
Hong-Ling TAN
;
Xiao-Qin LU
;
Zhi DONG
;
Yue GAO
Author Information
1. Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
- Publication Type:Journal Article
- MeSH:
Adenine;
pharmacology;
Apoptosis;
drug effects;
radiation effects;
Caspase 9;
genetics;
metabolism;
Cell Line;
Cell Survival;
drug effects;
radiation effects;
Gamma Rays;
Gene Expression;
drug effects;
radiation effects;
Humans;
Lymphocytes;
drug effects;
radiation effects;
Proto-Oncogene Proteins c-bcl-2;
genetics;
metabolism;
Radiation-Protective Agents;
pharmacology;
bcl-2-Associated X Protein;
genetics;
metabolism
- From:
Chinese Journal of Natural Medicines (English Ed.)
2013;11(2):139-144
- CountryChina
- Language:English
-
Abstract:
AIM:To investigate the radioprotective effect of adenine on irradiated lymphocytes and discover the possible mechanisms of protection.
METHODS:Lymphocytes were pretreated for 12 h with adenine (0.001-0.1 μmol·L(-1)) and then exposed to 4 Gy radiation. Cell viability was observed by the MTS assay, apoptosis was detected by Annexin V-FITC/PI, DNA ladder, and caspase 3/7 activity. Caspase-9, Bax, and Bcl-2 gene expression was investigated by RT-PCR.
RESULTS:Irradiation increased cell death and DNA fragmentation. Pretreatment with adenine significantly reversed this tendency. Furthermore, several anti-apoptotic characteristics of adenine were determined, including the ability to inhibit caspase 3/7, upregulate B-cell lymphoma (Bcl-2) and downregulate Bcl-2- associated X (Bax), capase-9 gene expression in 4 Gy-irradiated AHH-1 cells.
CONCLUSION:The results suggest that adenine had a radioprotective effect to inhibit apoptosis in a concentration dependent manner.
