Isolation of an anti-complementary polysaccharide from the root of Bupleurum chinense and identification of its targets in complement activation cascade.
10.1016/S1875-5364(13)60046-1
- Author:
Hong-Ye DI
1
;
Yun-Yi ZHANG
;
Dao-Feng CHEN
Author Information
1. School of Pharmacy, Fudan University, Shanghai 201203, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Bupleurum;
chemistry;
Carbohydrate Sequence;
Complement Activation;
drug effects;
Complement Inactivating Agents;
chemistry;
isolation & purification;
pharmacology;
Hemolysis;
drug effects;
Humans;
Male;
Molecular Weight;
Plant Extracts;
chemistry;
isolation & purification;
pharmacology;
Plant Roots;
chemistry;
Polysaccharides;
chemistry;
isolation & purification;
pharmacology
- From:
Chinese Journal of Natural Medicines (English Ed.)
2013;11(2):177-184
- CountryChina
- Language:English
-
Abstract:
AIM:To isolate and characterize the anti-complementary polysaccharide from the root of Bupleurum chinense.
METHODS:Bioactivity-guided fractionation and purification was used to obtain the anti-complementary polysaccharide from the hot-water extract of the root of Bupleurum chinense. The polysaccharide was characterized by various chemical and spectral analyses. The anti-complementary activities were evaluated by hemolytic assay in vitro. The action targets were identified in the system with individual complement-depleted sera.
RESULTS:A homogeneous polysaccharide BC-PS2 was isolated as an anti-complementary agent. It was identified as a branched polysaccharide with an average molecular weight about 2 000 KDa, composed of Glc, Ara, Gal, and Man in the ratio 3.5 : 2.4 : 2.0 : 1.0, respectively, along with a trace of Rha and Xyl, and only 1.11% of protein. The main linkages of the residues of BC-PS2 include terminal, 1, 6-linked, 1, 3-linked and 1, 3, 6-linked Glcp, terminal and 1, 5-linked Araf, terminal, 1, 4-linked, 1, 6-linked and 1, 4, 6-linked Galp, terminal, and, 1, 4-linked and 1, 4, 6-linked Manp. The bioassay experiments revealed that BC-PS2 inhibited complement activation on both the classical and alternative pathways, with CH50 and AP50 of (0.222 ± 0.013) and (0.356 ± 0.032) mg·mL(-1), respectively. Preliminary mechanism studies indicated that BC-PS2 interacted with C1q, C2, and C9 components.
CONCLUSION:The results demonstrated that BC-PS2 is an anti-complementary polysaccharide, and should be important constituent of the root of Bupleurum chinense for its application in the treatment of diseases associated with the excessive activation of complement system.
