A rapid and sensitive liquid chromatography-tandem mass spectrometric method for determination of actinoside E in rat plasma and application to a pharmacokinetic study.
10.1016/S1875-5364(13)60064-3
- Author:
Li-Ping QU
1
;
Yong-Hua SU
;
Guo-Yin ZHENG
;
Hai-Liang XIN
;
Chang-Quan LING
Author Information
1. Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
- Publication Type:Journal Article
- Keywords:
Actinoside E;
LC-MS/MS;
Pharmacokinetics;
Rat plasma
- MeSH:
Actinidia;
chemistry;
Animals;
Chromatography, High Pressure Liquid;
methods;
Glycosides;
blood;
pharmacokinetics;
Kaempferols;
blood;
pharmacokinetics;
Male;
Plant Extracts;
blood;
pharmacokinetics;
Rats;
Rats, Sprague-Dawley;
Sensitivity and Specificity;
Tandem Mass Spectrometry;
methods
- From:
Chinese Journal of Natural Medicines (English Ed.)
2013;11(4):427-432
- CountryChina
- Language:English
-
Abstract:
A highly sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed for the determination of actinoside E in rat plasma. The analytes were extracted by ethyl acetate and an analogue of actinoside F was used as the internal standard. The mobile phase consisted of methanol-water (50: 50, V/V) containing 0.1% formic acid was delivered at a flow rate of 0.3 mL·min(-1) to a Zorbax SB-C18 column (100 mm × 2.1 mm, 3.5 μm). The detection was performed by electrospray ionization mass spectrometry in the negative multiple reaction monitoring mode with a chromatograph run time of 3.0 min. Calibration curves of actinoside E were linear in the range of 0.5-2 500 ng·mL(-1). In this range, intra- and inter-day precision ranged from 1.7% to 7.5% and 2.0% to 8.9%, respectively. The accuracy ranged from 95.7% to 108.6%, and extraction recovery from 83.2% to 85.5%. This method was successfully applied to a pharmacokinetic study of actinoside E in rats after intravenous (5 mg·kg(-1)) and oral (100 mg·kg(-1)) administration, and the results showed that actinoside E was poorly absorbed with an absolute bioavailability being approximately 0.27%.
