A new diphenyl ether from the endophytic fungus Verticillium sp. isolated from Rehmannia glutinosa.
10.1016/S1875-5364(13)60078-3
- Author:
Wei PENG
1
,
2
;
Fei YOU
3
;
Xiao-Li LI
4
;
Min JIA
3
;
Cheng-Jian ZHENG
3
;
Ting HAN
5
;
Lu-Ping QIN
1
,
6
Author Information
1. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
2. Department of Pharmacology, College of Pharmacy, Third Military Medical University, Chongqing 400038, China.
3. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
4. Department of Pharmacology, College of Pharmacy, Third Military Medical University, Chongqing 400038, China.
5. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China. Electronic address: hanting@smmu.edu.cn.
6. Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai 200433, China. Electronic address: qinsmmu@126.com.
- Publication Type:Journal Article
- Keywords:
Antifungal activities;
Cytotoxic activity;
Diphenyl ether;
Endophytic fungus;
Rehmannia glutinosa
- MeSH:
Antifungal Agents;
chemistry;
isolation & purification;
metabolism;
pharmacokinetics;
Antineoplastic Agents;
chemistry;
isolation & purification;
metabolism;
pharmacokinetics;
Aspergillus fumigatus;
drug effects;
Candida albicans;
drug effects;
Cell Line, Tumor;
Endophytes;
chemistry;
metabolism;
Humans;
Phenyl Ethers;
chemistry;
isolation & purification;
metabolism;
pharmacokinetics;
Rehmannia;
microbiology;
Verticillium;
chemistry;
metabolism
- From:
Chinese Journal of Natural Medicines (English Ed.)
2013;11(6):673-675
- CountryChina
- Language:English
-
Abstract:
AIM:To investigate the chemical constituents of the endophytic fungus Verticillium sp. isolated from Rehmannia glutinosa.
METHODS:The compounds were isolated and purified by repeated column chromatography, and their structures were determined on the basis of physicochemical properties and spectral analysis. Their cytotoxic and antifungal activities were evaluated.
RESULTS:Ten compounds were obtained and their structures were identified as 2, 4-dihydroxy-2', 6-diacetoxy-3'-methoxy-5'-methyl-diphenyl ether (1), paecilospirone (2), α-acetylorcinol (3), 2-methoxy-1,8-dimethyl-xanthen-9-one (4), 4-hydroxy-α-lapachone (5), enalin A (6), 2,3,4-trimethyl-5,7-dihydroxy-2,3-dihydrobenzofuran (7), 4-hydroxyethyl-phenol (8), 2,4-dihydroxy-3,5,6-trimethyl- methylbenzoate (9), and 3-isopropenyl-(Z)-monomethyl maleate (10).
CONCLUSIONS:Compound 1 is a new diphenyl ether, and showed cytotoxic activity against HL-60 cells (IC50 2.24 μg · mL(-1)), and antifungal activities against Candida albicans (MIC 8 μg · mL(-1)) and Aspergillus fumigatus (MIC 16 μg · mL(-1)).
