In vivo anti-inflammatory activity of Tabernaemontana divaricata leaf extract on male albino mice.
10.1016/S1875-5364(13)60086-2
- Author:
Sachin JAIN
1
;
Praveen SHARMA
2
;
Santosh GHULE
2
;
Ankit JAIN
2
;
Nitesh JAIN
3
Author Information
1. College of Pharmacy, IPS Academy, Rajendra Nagar, AB Road, Indore MP India 452012. Electronic address: sachin225819@rediffmail.com.
2. College of Pharmacy, IPS Academy, Rajendra Nagar, AB Road, Indore MP India 452012.
3. Department of Pharmacy, Oriental University, Indore MP India 452001.
- Publication Type:Journal Article
- Keywords:
Anti-inflammatory;
Mouse ear edema;
Plant extract;
Quercitrin;
Tabernaemontana divaricata;
Traditional medicine
- MeSH:
Animals;
Anti-Inflammatory Agents;
administration & dosage;
Edema;
drug therapy;
Humans;
Male;
Mice;
Plant Extracts;
administration & dosage;
Plant Leaves;
chemistry;
Tabernaemontana;
chemistry
- From:
Chinese Journal of Natural Medicines (English Ed.)
2013;11(5):472-476
- CountryChina
- Language:English
-
Abstract:
AIM:To study the in vivo anti-inflammatory activity of Tabernaemontana divaricata leaf extract on male albino mice.
METHODS:Aqueous decoction and methanol leaf extracts were tested for their ability to reduce croton oil-induced edema in the mouse ear after topical application. The methanol leaf extract dose-dependently inhibited the croton oil-induced ear edema in mice (ID50 <500 μg·cm(-2)). A bioassay-guided liquid-liquid fractionation of this methanol extract gave four active fractions: water insoluble (F1), hexane (F2), ethyl acetate (F3) and water (F4).
RESULTS:The hexane fraction showed a very high activity (42.1% inhibition at 0.7 μg·cm(-2)) as compared to the control. The other fractions were less active (F1: 56.1% at 506.2 μg·cm(-2); F3: 57.3% at 289.3 μg·cm(-2); and F4: 31.9% for 203.8 μg·cm(-2)) while indomethacin gave 48.8% of inhibition at 90 μg·cm(-2). The activity of F1 and F3 may be at least in part explained by the presence of anti-inflammatory flavonoids, while the activity was not correlated to the tannin contents. No compounds were detected in the most active F2 fraction.
CONCLUSIONS:The results give a rational support to the traditional use of T. divaricata in tropical India as anti-inflammatory agent.
