H1-A, a compound isolated from Fusarium oxysporum inhibits hepatitis C virus (HCV) NS3 serine protease.
10.1016/S1875-5364(16)30031-0
- Author:
Li-Yuan YANG
1
,
2
;
Jun LIN
3
;
Bin ZHOU
3
;
Yan-Gang LIU
4
;
Bao-Quan ZHU
1
,
5
Author Information
1. School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China
2. State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 201203, China.
3. State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 201203, China.
4. School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: ygliu@sjtu.edu.cn.
5. State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 201203, China. Electronic address: baoquanzhu@126.com.
- Publication Type:Journal Article
- Keywords:
Bioactive compound;
Fusarium oxysporum;
H1-A
- MeSH:
Enzyme Inhibitors;
chemistry;
isolation & purification;
metabolism;
Fusarium;
chemistry;
metabolism;
Hepacivirus;
drug effects;
enzymology;
genetics;
Hepatitis C;
virology;
Humans;
Magnetic Resonance Spectroscopy;
Viral Nonstructural Proteins;
antagonists & inhibitors;
metabolism
- From:
Chinese Journal of Natural Medicines (English Ed.)
2016;14(4):299-302
- CountryChina
- Language:English
-
Abstract:
The present study was aimed to isolate the active compounds from the fermentation products of Fusarium oxysporum, which had hepatitis C virus (HCV) NS3 protease inhibitory activity. A bioactive compound was isolated by reverse-phase silica-gel column chromatography, silica-gel column chromatography, semi-preparative reverse-phase High Performance Liquid Chromatography (HPLC), and then its molecular structure was elucidated based on the spectrosopic analysis. As a result, the compound (H1-A, 1) Ergosta-5, 8 (14), 22-trien-7-one, 3-hydroxy-,(3β, 22E) was isolated and identified. To the best of our knowledge, this was the first report on the isolation of H1-A from microorganisms with the inhibitory activity of NS3 protease.
