Anti-proliferative and apoptotic effects of S1, a tetrandrine derivative, in human gastric cancer BGC-823 cells.
10.1016/S1875-5364(16)30062-0
- Author:
Rong-Rong LEI
1
;
Hai-Feng HU
2
,
3
;
Fan BAI
4
;
Ying LIU
2
,
3
;
Chun-Zhen WU
2
,
5
;
Xiao-Xing HUANG
1
;
Li-Ping XIE
1
;
You-Jia HU
1
Author Information
1. Shanghai Institute of Pharmaceutical Industry, Shanghai 200040, China.
2. Shanghai Institute of Pharmaceutical Industry, Shanghai 200040, China
3. Sinopharm Health Industry Research Co., Ltd., Shanghai 200040, China.
4. The Kochi Prefectural Makino Botanical Garden, Kochi 4200-6, Japan.
5. Sinopharm Health Industry Research Co., Ltd., Shanghai 200040, China. Electronic address: czw1962@126.com.
- Publication Type:Journal Article
- Keywords:
Apoptosis;
BGC-823 cells;
G2/M phase arrest;
Tetrandrine derivative
- MeSH:
Antineoplastic Agents;
pharmacology;
Apoptosis;
drug effects;
Benzylisoquinolines;
chemistry;
pharmacology;
Caspase 3;
genetics;
metabolism;
Caspase 9;
genetics;
metabolism;
Cell Cycle Checkpoints;
drug effects;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Cell Survival;
drug effects;
Humans;
Proto-Oncogene Proteins c-bcl-2;
genetics;
metabolism;
Stomach Neoplasms;
drug therapy;
enzymology;
genetics;
physiopathology;
bcl-2-Associated X Protein;
genetics;
metabolism
- From:
Chinese Journal of Natural Medicines (English Ed.)
2016;14(7):527-533
- CountryChina
- Language:English
-
Abstract:
The aim of the study was to investigate the anti-proliferation and apoptosis-inducing effects of S1, a novel tetrandrine derivative, in human gastric cancer BGC-823 cells and explore the possible mechanism of action. The anti-proliferative activity was determined by MTT assay; the induction of cell cycle arrest and apoptosis were detected by flow cytometry. Quantitative real time RT-PCR and Western blotting were used to evaluate the mRNA and protein expression levels in mitochondrial pathway. S1 significantly reduced cell viability and induced a G2/M phase arrest and apoptosis in dose- and time-dependent manner. Further studies showed that S1 increased mRNA and protein expression of Bax and the Bax/Bcl-2 ratio. Moreover, S1 decreased the protein expression of procaspase-9 and procaspase-3, suggesting that the induction of apoptosis may be related to the alteration of the ratio of Bax/Bcl-2 and the activation of caspases. These findings suggested that S1 merits further investigation as a novel therapeutic agent for the treatment of human gastric cancer.
