Identification and characterization of a novel neuropeptide (neuropeptide Y-HS) from leech salivary gland of Haemadipsa sylvestris.
10.1016/S1875-5364(16)30080-2
- Author:
Wei-Hui LIU
1
;
Yan CHEN
1
;
Xue-Wei BAI
1
;
Hui-Min YAO
1
;
Xu-Guang ZHANG
2
;
Xiu-Wen YAN
3
;
Ren LAI
4
Author Information
1. Life Sciences College, Nanjing Agricultural University, Nanjing 210095, China.
2. Major Cardiovascular Surgery, Kunming Yanan Hospital, Kunming 650051, China.
3. Life Sciences College, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: yanxw@njau.edu.cn.
4. Life Sciences College, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: rlai72@njau.edu.cn.
- Publication Type:Journal Article
- Keywords:
Anti-inflammation;
Haemadipsa sylvestris;
Leech;
Neuropeptide Y;
Salivary gland
- MeSH:
Amino Acid Sequence;
Animals;
Immunologic Factors;
administration & dosage;
chemistry;
genetics;
Inflammation;
drug therapy;
immunology;
Interferon-gamma;
immunology;
Interleukin-6;
immunology;
Leeches;
chemistry;
Mass Spectrometry;
Mice;
Molecular Sequence Data;
Neuropeptide Y;
administration & dosage;
chemistry;
genetics;
Peptide Mapping;
Salivary Glands;
chemistry;
Tumor Necrosis Factor-alpha;
immunology
- From:
Chinese Journal of Natural Medicines (English Ed.)
2016;14(9):677-682
- CountryChina
- Language:English
-
Abstract:
The present study was designed to identify immunomodulatory components from the leech salivary gland of Haemadipsa sylvestris. The Sephadex G-50, Resource(TM) S column chromatography and reverse-phase high performance liquid chromatography (RP-HPLC) were used to isolate and purify the salivary gland extracts (SGE). Structural analysis of isolated compounds was based on Edman degradation and matrix assisted laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS). The cDNA encoding the precursor of the compound was cloned from the cDNA library of the salivary gland of H. sylvestris. The levels of inflammatory mediators, including tumor necrosis factor-α (TNF-α), interferon γ (IFN-γ), interleukin-6 (IL-6), and monocyte chemotactic protein-1 (MCP-1) were assayed using an enzyme-linked immunosorbent assay (ELISA). The effects on cell proliferation and cell viability were observed using MTT assay. A novel neuropeptide Y (Neuropeptide Y-HS) from the leech salivary gland of H. sylvestris was purified and characterized. It was composed of 36 amino acid residues and the amino acid sequence was determined to be FLEPPERPAVFTSVEQMKSYIKALNDYYLLLGRPRF-NH2, containing an amidated C-terminus. It showed significant inhibitory effects on the production of inflammatory cytokines including TNF-α, IFN-γ, IL-6, and MCP-1. Neuropeptide Y was identified from leeches for the first time. The presence of neuropeptide Y-HS in leech salivary gland may help get blood meal from hosts and inhibit inflammation.
