LC-MS/MS analysis and pharmacokinetic study on five bioactive constituents of Tanreqing injection in rats.
10.1016/S1875-5364(16)30091-7
- Author:
Feng ZHANG
1
;
Liang SUN
2
,
3
,
4
;
Shou-Hong GAO
1
;
Wan-Sheng CHEN
5
;
Yi-Feng CHAI
6
Author Information
1. Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
2. Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
3. Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
4. Department of Pharmacy, The 98th Hospital of PLA, HuZhou 313000, China.
5. Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China. Electronic address: chenwansheng@smmu.edu.cn.
6. Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, China. Electronic address: yfchai@smmu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Bile acids;
Flavones;
Pharmacokinetics;
Phenolic acids;
Tanreqing Injection
- MeSH:
Animals;
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
administration & dosage;
chemistry;
pharmacokinetics;
Male;
Molecular Structure;
Rats;
Rats, Sprague-Dawley;
Tandem Mass Spectrometry;
methods
- From:
Chinese Journal of Natural Medicines (English Ed.)
2016;14(10):769-775
- CountryChina
- Language:English
-
Abstract:
Tanreqing injection (TRQ), a well-known traditional Chinese medicine formula, is commonly used to treat respiratory diseases. In the present study, a rapid, selective, and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to simultaneously determinate the plasma contents of 5 major constituents of TRQ, including chlorogenic acid (CHA), caffeic acid (CFA), baicalin (BA), ursodeoxycholic acid (UDCA) and chenodeoxycholic acid (CDCA) in rats after intravenous administration of TRQ. Chromatographic separation was performed on an Agilent Zorbax SB-C column (3.5 μm, 100 mm × 2.1 mm), with acetonitrile and 0.1% aqueous formic acid as mobile phase at a flow rate of 0.3 mL·min. The calibration curves were linear over the ranges of 27.0-13 333.0 ng·mL for CFA, 30.0-14 933.0 ng·mL for CHA, 50.0-50 333.0 ng·mL for BA, 550.0-55 000.0 ng·mL for UDCA, and 480.0-48 000.0 ng·mL for CDCA, respectively. Intra- and inter-day precisions (relative standard deviations, RSDs) were from 3.11% to 14.08%. The extraction recoveries were greater than 71% and accuracy (relative recovery) was from 89% to 137% for all analytes, except endogenous bile acids. This validated method was successfully applied to the first pharmacokinetic study of CFA, CHA, BA, UDCA and CDCA in rat plasma after intravenous administration of TRQ.
