ITS2 barcoding DNA region combined with high resolution melting (HRM) analysis of Hyoscyami Semen, the mature seed of Hyoscyamus niger.
10.1016/S1875-5364(17)30014-6
- Author:
Chao XIONG
1
,
2
;
Zhi-Gang HU
1
,
2
;
Yuan TU
1
,
3
,
4
;
He-Gang LIU
5
;
Ping WANG
5
;
Ming-Ming ZHAO
6
;
Yu-Hua SHII
6
;
Lan WU
1
,
2
;
Wei SUN
7
;
Shi-Lin CHEN
1
,
8
Author Information
1. College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
3. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
4. Traditional Chinese Medicine Resources Research Center, Tianjin Tasly Pharmaceutical Co., Ltd., Tianjin 300402, China.
5. College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China.
6. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
7. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. Electronic address: wsun@icmm.ac.cn.
8. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. Electronic address: slchen@icmm.ac.cn.
- Publication Type:Journal Article
- Keywords:
Bar-HRM;
Clinical safe medication;
Hyoscyami Semen;
Identification
- MeSH:
China;
DNA Barcoding, Taxonomic;
methods;
DNA, Intergenic;
chemistry;
genetics;
DNA, Plant;
chemistry;
genetics;
Discriminant Analysis;
Drug Contamination;
Drugs, Chinese Herbal;
chemistry;
Hyoscyamus;
genetics;
growth & development;
Seeds;
genetics;
growth & development;
Transition Temperature
- From:
Chinese Journal of Natural Medicines (English Ed.)
2016;14(12):898-903
- CountryChina
- Language:English
-
Abstract:
Hyoscyami Semen, the mature dried seed of Hyoscyamus niger L., has long been used as a traditional Chinese medicine to treat human diseases. Hyoscyami Semen is found in local markets in China. In markets, sellers and buyers commonly inadvertently mix the seeds of H. niger with the seeds of related species such as Hygrophila salicifolia (Vahl) Nees, Astragalus complanatus R. Br., Cuscuta australis R. Br., Cuscuta chinensis Lam., and Impatiens balsamina L. because of their similar morphologies or similar names. Thus, developing a reliable method for discriminating H. niger seeds from its adulterants is necessary to reduce confusion and ensure the safe use of Hyoscyami Semen. The present study was designed to evaluate the efficiency of high-resolution melting analysis combined with DNA barcoding (Bar-HRM) with internal transcribed spacer 2 to discriminate H. niger. Our results show that Bar-HRM successfully identified the adulterants and detected the proportion of H. niger DNA extract within an admixture. In particular, HRM detected H. niger DNA extract in A. complanatus DNA extract at concentrations as low as 1%. In conclusion, the Bar-HRM method developed in the present study for authenticating H. niger is rapid and cost-effective. It can be used in the future to guarantee the purity of Hyoscyami Semen for the clinical use.
