Thymoquinone inhibits the migration of mouse neuroblastoma (Neuro-2a) cells by down-regulating MMP-2 and MMP-9.
10.1016/S1875-5364(17)30015-8
- Author:
Paramasivam ARUMUGAM
1
;
Raghunandhakumar SUBRAMANIAN
2
;
Jayaseelan Vijayashree PRIYADHARSINI
3
;
Jayaraman GOPALSWAMY
3
Author Information
1. Department of Genetics, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Sekkizhar campus, Taramani, Chennai-600113, India. Electronic address: paramasivam0103@gmail.com.
2. Department of Biochemistry, University of Madras, Guindy Campus, Chennai-600025, India.
3. Department of Genetics, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Sekkizhar campus, Taramani, Chennai-600113, India.
- Publication Type:Journal Article
- Keywords:
MMP-2;
Migration;
NF-κB;
Neuroblastoma;
Thymoquinone
- MeSH:
Animals;
Apoptosis;
drug effects;
Benzoquinones;
pharmacology;
Cell Line, Tumor;
Down-Regulation;
drug effects;
Humans;
Matrix Metalloproteinase 2;
genetics;
metabolism;
Matrix Metalloproteinase 9;
genetics;
metabolism;
Mice;
Neuroblastoma;
drug therapy;
enzymology;
genetics;
physiopathology;
Nigella sativa;
chemistry;
Plant Extracts;
pharmacology
- From:
Chinese Journal of Natural Medicines (English Ed.)
2016;14(12):904-912
- CountryChina
- Language:English
-
Abstract:
Thymoquinone (TQ), an active component derived from the medial plant Nigella sativa, has been used for medical purposes for more than 2 000 years. Recent studies have reported that TQ blocked angiogenesis in animal model and reduced migration, adhesion, and invasion of glioblastoma cells. We have recently shown that TQ could exhibit a potent cytotoxic effect and induce apoptosis in mouse neuroblastoma (Neuro-2a) cells. In the present study, TQ treatment markedly decreased the adhesion and migration of Neuro-2a cells. TQ down-regulated MMP-2 and MMP-9 protein expression and mRNA levels and their activities. Furthermore, TQ significantly down-regulated the protein expression of transcription factor NF-κB (p65) but not significantly altered the expression of N-Myc. Taken together, our data indicated that TQ's inhibitory effect on the migration of Neuro-2a cells was mediated through the suppression of MMP-2 and MMP-9 expression, suggesting that TQ treatment can be a promising therapeutic strategy for human malignant neuroblastoma.
