Development of specific and quantitative methods for the quality control of the polysaccharides from sea-tangle and sargassum.
10.1016/S1875-5364(17)30022-5
- Author:
Hua-Li LONG
1
;
Jing-Xian ZHANG
1
;
Ji-Ling GUO
1
;
Guo-Qi SUI
1
;
Jin-Jun HOU
1
;
Wen-Zhi YANG
1
;
Su-Mei HAN
1
;
Wan-Ying WU
2
;
De-An GUO
1
Author Information
1. National Engineering Laboratory for TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
2. National Engineering Laboratory for TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. Electronic address: yxi@lsuhsc.edu.
- Publication Type:Journal Article
- Keywords:
Polysaccharides;
Quality control;
Sargassum;
Sea-tangle
- MeSH:
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
chemistry;
Laminaria;
chemistry;
Plant Extracts;
chemistry;
isolation & purification;
Polysaccharides;
chemistry;
Quality Control;
Sargassum;
chemistry;
Seaweed;
chemistry
- From:
Chinese Journal of Natural Medicines (English Ed.)
2016;14(12):954-960
- CountryChina
- Language:English
-
Abstract:
Polysaccharides from numerous traditional Chinese medicines have been proven as the bioactive ingredients and are hence used as the quality control markers. However, the assessment criteria always show a poor specificity, due to the lack of systematic comparison among the analogous herbs. In the present study, two similar materials, namely sea-tangle and sargassum, were selected as the model herbs to develop more specific methods for quality control. Two well-established methods, determination of the total polysaccharides content and monosaccharides composition analysis, were both employed. Based upon the quantitative results, the evaluation criteria of the polysaccharides contents of not less than 2.0% and 1.7% were proposed for sea-tangle and sargassum, respectively. Nine identical monosaccharide derivatives appeared on the HPLC chromatograms of the hydrolysis and derivatized solutions of the two drugs. Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components. A discriminative criterion using the peak area ratio of these two monosaccharides derivatives was proposed for the qualitative identification. In conclusion, a more specific and quantitative quality control method was developed for sea-tangle and sargassum.
