Anti-asthmatic effects of oxymatrine in a mouse model of allergic asthma through regulating CD40 signaling.
10.1016/S1875-5364(15)30028-5
- Author:
Tian-Zhu ZHANG
1
;
Qiang FU
2
;
Tong CHEN
3
;
Shi-Ping MA
4
Author Information
1. School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun 130117, China.
2. Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, China.
3. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
4. Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, China. Electronic address: mashipingbest@126.com.
- Publication Type:Journal Article
- Keywords:
Asthma;
CD40;
Oxymatrine
- MeSH:
Alkaloids;
pharmacology;
Animals;
Anti-Asthmatic Agents;
pharmacology;
Anti-Inflammatory Agents;
pharmacology;
Asthma;
drug therapy;
Bronchoalveolar Lavage Fluid;
chemistry;
CD40 Antigens;
metabolism;
Dexamethasone;
pharmacology;
Disease Models, Animal;
Enzyme-Linked Immunosorbent Assay;
Female;
Immunoglobulin E;
metabolism;
Interleukins;
metabolism;
Irritants;
toxicity;
Mice, Inbred BALB C;
Ovalbumin;
toxicity;
Pulmonary Eosinophilia;
chemically induced;
drug therapy;
Quinolizines;
pharmacology;
Random Allocation;
Signal Transduction;
drug effects
- From:
Chinese Journal of Natural Medicines (English Ed.)
2015;13(5):368-374
- CountryChina
- Language:English
-
Abstract:
The aim of the study was to investigate the anti-asthmatic effects of oxymatrine (OXY) and the possible underlying mechanisms. The mouse asthma model was established by ovalbumin (OVA) intraperitoneal injection. A total of fifty mice were randomly assigned to five groups: control, OVA, OVA + dexamethasone (Dex, 2 mg · kg(-1)), and OVA + OXY (40 mg · kg(-1)), and OVA + OXY (80 mg · kg(-1)), respectively. Histological studies were conducted by the hematoxylin and eosin (HE) staining, the levels of interleukin-4 (IL-4), interleukin-5 (IL-5), interleukin-13, and IgE were evaluated by enzyme-linked immunosorbent assay (ELISA), and the protein level of CD40 was analyzed by Western blotting. OXY inhibited OVA-induced increases in eosinophil count; the levels of IL-4, IL-5, IgE, and IL-13 were recovered. It also substantially inhibited OVA-induced eosinophilia in lung tissues and the expression of CD40 protein. These findings suggest that OXY may effectively ameliorate the progression of asthma and could be explored as a possible therapy for patients with allergic asthma.
