Diagnostic ion filtering strategy for chemical characterization of Guge Fengtong Tablet with high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry.
10.1016/S1875-5364(15)30031-5
- Author:
Su-Ling ZENG
1
;
Xin-Guang LIU
1
;
Chang-Jiang-Sheng LAI
1
;
E-Hu LIU
2
;
Ping LI
3
Author Information
1. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
2. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: liuehu2011@163.com.
3. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: liping2004@126.com.
- Publication Type:Journal Article
- Keywords:
Diagnostic ion filtering strategy;
Guge Fengtong Tablet;
HPLC-ESI-Q-TOF/MS
- MeSH:
Catechols;
isolation & purification;
Chromatography, High Pressure Liquid;
Diarylheptanoids;
isolation & purification;
Drugs, Chinese Herbal;
chemistry;
Fatty Alcohols;
isolation & purification;
Hydroxybenzoates;
isolation & purification;
Saponins;
isolation & purification;
Spectrometry, Mass, Electrospray Ionization;
Tablets, Enteric-Coated;
chemistry;
Tandem Mass Spectrometry
- From:
Chinese Journal of Natural Medicines (English Ed.)
2015;13(5):390-400
- CountryChina
- Language:English
-
Abstract:
The present study was designed to characterize the chemical constituents of Guge Fengtong Tablet (GGFTT). Based on the chromatographic retention behavior, fragmentation pathways of chemical components and the published literatures, a diagnostic ion filtering strategy with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (HPLC-ESI-Q-TOF/MS) was established to identify the multiple bioactive constituents of GGFTT. The rapid identification of forty-seven components, including 18 phenolic acids, 8 saponins, 14 gingerol-related compounds, and 7 diarylhepatonoids, was accomplished using this newly developed method. The coupling of HPLC-ESI-Q-TOF/MS with the diagnostic ion filtering strategy was useful and efficient for the in-depth structural elucidation of chemical compounds of GGFTT.
