A rapid method for chemical fingerprint analysis of Pan Panax notoginseng powders by ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.
10.1016/S1875-5364(15)30042-X
- Author:
Peng LIU
1
;
He-Shuil YU
2
;
Li-Juan ZHANG
3
,
4
;
Xin-Bo SONG
1
;
Li-Ping KANG
2
;
Jing-Yuan LIU
2
;
Jie ZHANG
2
;
Man CAO
1
;
Kate YU
5
;
Ting-Guo KANG
6
;
Bai-Ping MA
7
Author Information
1. Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
2. Beijing Institute of Radiation Medicine, Beijing 100850, China.
3. Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
4. College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China.
5. Waters Corporation, Milford, MA 01757, USA.
6. College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China.
7. Beijing Institute of Radiation Medicine, Beijing 100850, China. Electronic address: mabaiping@sina.com.
- Publication Type:Journal Article
- Keywords:
Chemical fingerprint;
Ginsenosides;
Notoginsenosides;
Panax notoginseng powder;
UPLC/Qtof MS(E)
- MeSH:
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
chemistry;
Panax notoginseng;
chemistry;
Powders;
chemistry;
Spectrometry, Mass, Electrospray Ionization;
methods
- From:
Chinese Journal of Natural Medicines (English Ed.)
2015;13(6):471-480
- CountryChina
- Language:English
-
Abstract:
A method coupling ultra-performance liquid chromatography (UPLC) with quadrupole time-of-flight mass spectrometer (Qtof MS) using the electrospray ionization (ESI) source was developed for the identification of the major saponins from Panax notoginseng powder (PNP). Ten different PNP samples were analyzed and evaluated for their quality by similarity evaluation and principle component analysis (PCA). Based on the accurate mass, summarized characteristic fragmentation behaviors, retention times of different types of saponins, related botanical biogenesis, and reported chromatographic behavior of saponins, fifty-one common peaks were effectively separated and identified, including 28 protopanaxadiol saponins and 18 protopanaxatriol saponins. Simultaneously, 15 significant discrepancy compounds were identified from the disqualified PNP samples. The established UPLC/Qtof MS fingerprint method was successfully applied for profiling and identifying the major saponins of PNP, providing a fast quality evaluation tool for distinguishing the authentic PNP and the adulterated products.
