Screening of natural compounds with neuronal differentiation promoting effects in a cell-based model.
10.1016/S1875-5364(15)30056-X
- Author:
Tao CHEN
1
;
Juan WANG
1
;
Mei LIU
1
;
Lu-Yong ZHANG
2
;
Hong LIAO
3
Author Information
1. National Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China.
2. National Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China. Electronic address: drugscreen@126.com.
3. National Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China. Electronic address: hliao@cpu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Drug screening model;
Neuronal differentiation;
P19 embryonic carcinoma cells;
Traditional Chinese Medicine;
Wnt signaling pathway
- MeSH:
Animals;
Biphenyl Compounds;
pharmacology;
Cell Differentiation;
drug effects;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Drug Evaluation, Preclinical;
methods;
Drugs, Chinese Herbal;
pharmacology;
Embryonal Carcinoma Stem Cells;
drug effects;
Lignans;
pharmacology;
Mice;
Neurons;
drug effects;
Quercetin;
analogs & derivatives;
pharmacology;
Tretinoin;
physiology;
Wnt Signaling Pathway
- From:
Chinese Journal of Natural Medicines (English Ed.)
2015;13(8):602-608
- CountryChina
- Language:English
-
Abstract:
The purpose of this study was to establish a drug screening method for small molecules extracted from traditional Chinese medicines (TCM) that have neuronal differentiation promoting effects, using P19 embryonic carcinoma cell as a cell-based model. First, the constructed plasmid (pTα1-Luc) was transfected into P19 cells to establish a screening model. Second, several TCMs were screened using the established model and all-trans-retinoic acid as a positive control. Finally, the underlying molecular mechanism was explored using immunofluorescence staining, qT-PCR, and Western blot analysis. Our results indicated that the drug screen model was established successfully and that both honokiol and hyperoside induced P19 differentiation into neurons, with the possible molecular mechanism being modulating the Wnt signaling pathway. In conclusion, the drug screening model developed in the present study provides a rapid, cell-based screening platform for identifying natural compounds with neuronal differentiation effects.
