Identification of maca (Lepidium meyenii Walp.) and its adulterants by a DNA-barcoding approach based on the ITS sequence.
10.1016/S1875-5364(15)30062-5
- Author:
Jin-Jin CHEN
1
,
2
;
Qing-Sheng ZHAO
3
;
Yi-Lan LIU
1
,
2
;
Sheng-Hua ZHA
1
,
4
,
5
;
Bing ZHAO
6
Author Information
1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
2. University of Chinese Academy of Sciences, Beijing 100049, China.
3. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
4. University of Chinese Academy of Sciences, Beijing 100049, China
5. Beijing Tong Ren Tang Health Pharmaceutical Co., Ltd., Beijing 100062, China.
6. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: bzhao@ipe.ac.cn.
- Publication Type:Journal Article
- Keywords:
DNA-barcoding;
ITS;
Lepidium meyenii;
Maca;
Molecular identification
- MeSH:
DNA Barcoding, Taxonomic;
methods;
DNA, Intergenic;
analysis;
DNA, Plant;
analysis;
Drug Contamination;
prevention & control;
Humans;
Lepidium;
genetics;
Phytotherapy
- From:
Chinese Journal of Natural Medicines (English Ed.)
2015;13(9):653-659
- CountryChina
- Language:English
-
Abstract:
Maca (Lepidium meyenii) is an herbaceous plant that grows in high plateaus and has been used as both food and folk medicine for centuries because of its benefits to human health. In the present study, ITS (internal transcribed spacer) sequences of forty-three maca samples, collected from different regions or vendors, were amplified and analyzed. The ITS sequences of nineteen potential adulterants of maca were also collected and analyzed. The results indicated that the ITS sequence of maca was consistent in all samples and unique when compared with its adulterants. Therefore, this DNA-barcoding approach based on the ITS sequence can be used for the molecular identification of maca and its adulterants.
