Quercetin promotes neurite growth through enhancing intracellular cAMP level and GAP-43 expression.
10.1016/S1875-5364(15)30064-9
- Author:
Ming-Ming CHEN
1
;
Zhi-Qi YIN
2
;
Lu-Yong ZHANG
3
;
Hong LIAO
4
Author Information
1. National Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China.
2. Department of Phytochemistry, China Pharmaceutical University, Nanjing 210009, China.
3. National Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China. Electronic address: drugscreen@126.com.
4. National Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China. Electronic address: hliao@cpu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Gap-43;
N1E-115;
Quercetin;
cAMP;
neurite growth
- MeSH:
Cell Line;
Cyclic AMP;
metabolism;
GAP-43 Protein;
metabolism;
Nerve Regeneration;
Neurites;
drug effects;
Plant Extracts;
pharmacology;
Quercetin;
pharmacology;
Signal Transduction
- From:
Chinese Journal of Natural Medicines (English Ed.)
2015;13(9):667-672
- CountryChina
- Language:English
-
Abstract:
The present study was designed to investigate the role of quercetin on neurite growth in N1E-115 cells and the underlying mechanisms. Quercetin was evaluated for its effects on cell numbers of neurites, neurite length, intracellular cAMP content, and Gap-43 expression in N1E-115 cells in vitro by use of microscopy, LANCE(tm) cAMP 384 kit, and Western blot analysis, respectively. Our results showed that quercetin could increase the neurite length in a concentration-dependent manner, but had no effect on the numbers of cells. Quercetin significantly increased the expression of cellular cAMP in a time- and concentration-dependent manner. The Gap-43 expression was up-regulated in a time-dependent manner. In conclusion, quercetin could promote neurite growth through increasing the intracellular cAMP level and Gap-43 expression.
