Pleurotus nebrodensis polysaccharide (PN-S) enhances the immunity of immunosuppressed mice.
10.1016/S1875-5364(15)30076-5
- Author:
Hai-Yan CUI
1
;
Chang-Lu WANG
2
;
Yu-Rong WANG
1
;
Zhen-Jing LI
1
;
Mian-Hua CHEN
1
;
Feng-Juan LI
1
;
Yan-Ping SUN
1
Author Information
1. Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
2. Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China. Electronic address: clw123@tust.edu.cn.
- Publication Type:Journal Article
- Keywords:
Cyclophosphamide;
Immunosuppressed mice;
Pleurotus nebrodensis polysaccharide
- MeSH:
Animals;
Antineoplastic Agents, Alkylating;
Biological Products;
pharmacology;
therapeutic use;
Cell Line;
Cyclophosphamide;
Immunity;
drug effects;
Immunologic Factors;
pharmacology;
therapeutic use;
Immunosuppression;
Interferon-gamma;
metabolism;
Interleukin-6;
metabolism;
Macrophages;
drug effects;
metabolism;
Male;
Mice, Inbred BALB C;
Neoplasms;
drug therapy;
immunology;
Nitric Oxide;
metabolism;
Nitric Oxide Synthase Type II;
metabolism;
Phagocytosis;
drug effects;
Pleurotus;
chemistry;
Polysaccharides;
pharmacology;
therapeutic use;
Tumor Necrosis Factor-alpha;
metabolism
- From:
Chinese Journal of Natural Medicines (English Ed.)
2015;13(10):760-766
- CountryChina
- Language:English
-
Abstract:
In the present study, the effects of Pleurotus nebrodensis polysaccharide (PN-S) on the immune functions of immunosuppressed mice were determined. The immunosuppressed mouse model was established by treating the mice with cyclophosphamide (40 mg/kg/2d, CY) through intraperitoneal injection. The results showed that PN-S administration significantly reversed the CY-induced weight loss, increased the thymic and splenic indices, and promoted proliferation of T lymphocyte, B lymphocyte, and macrophages. PN-S also enhanced the activity of natural killer cells and increased the immunoglobulin M (IgM) and immunoglobulin G (IgG) levels in the serum. In addition, PN-S treatment significantly increased the phagocytic activity of mouse peritoneal macrophages. PN-S also increased the levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interferon-γ (INF-γ), and nitric oxide (NOS) in splenocytes. qRT-PCR results also indicated that PN-S increased the mRNA expression of IL-6, TNF-α, INF-γ, and nitric oxide synthase (iNOS) in the splenocytes. These results suggest that PN-S treatment enhances the immune function of immunosuppressed mice. This study may provide a basis for the application of this fungus in adjacent immunopotentiating therapy against cancer and in the treatment of chemotherapy-induced immunosuppression.
