The importance of start codon of nosM in nosiheptide production.
10.1016/S1875-5364(15)30089-3
- Author:
Lei JIANG
1
;
Yan-Jiu XUE
1
;
Wei-Ying LIU
1
;
Min MA
1
;
Xu-Ri WU
1
;
Shu-Zhen WANG
2
;
Yi-Jun CHEN
3
Author Information
1. State Key Laboratory of Natural Medicines and Laboratory of Chemical Biology, China Pharmaceutical University, Nanjing 210009, China.
2. State Key Laboratory of Natural Medicines and Laboratory of Chemical Biology, China Pharmaceutical University, Nanjing 210009, China. Electronic address: shuzhenwang@cpu.edu.cn.
3. State Key Laboratory of Natural Medicines and Laboratory of Chemical Biology, China Pharmaceutical University, Nanjing 210009, China. Electronic address: yjchen@cpu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Biosynthesi;
Leader peptide;
Nosiheptide;
Start codon
- MeSH:
Anti-Bacterial Agents;
biosynthesis;
Chromatography, High Pressure Liquid;
Codon, Initiator;
Escherichia coli;
Genes, Bacterial;
Mutation;
Streptomyces;
genetics;
metabolism;
Thiazoles;
metabolism
- From:
Chinese Journal of Natural Medicines (English Ed.)
2015;13(11):854-860
- CountryChina
- Language:English
-
Abstract:
The present study was designed to investigate the effects of start codon of nosM on the biosynthesis of nosiheptide. Target genes were amplified by overlap PCR. After homologous recombination to construct engineered strains, nosiheptide production was analyzed by HPLC. Three mutants with different start codon of nosM were constructed, and nosiheptide production of each mutant was analyzed and compared. Replacement of the start codon of nosM significantly decreased the production of nosiheptide. In conclusion, start codon usage could greatly affect the biosynthetic efficiency in the biosynthetic gene cluster of nosiheptide.
