Diversity, bioactivities, and metabolic potentials of endophytic actinomycetes isolated from traditional medicinal plants in Sichuan, China.
10.1016/S1875-5364(15)30102-3
- Author:
Peng QIU
1
,
2
;
Zhi-Xiang FENG
1
,
3
;
Jie-Wei TIAN
1
,
2
;
Zu-Chao LEI
1
,
2
;
Lei WANG
1
,
2
;
Zhi-Gang ZENG
4
;
Yi-Wen CHU
4
;
Yong-Qiang TIAN
1
,
5
Author Information
1. Key laboratory of Leather Chemistry and Engineering, Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu, 610065, China
2. Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, China.
3. Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052, China.
4. Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052, China.
5. Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, China. Electronic address: yqtian@scu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Bioactivity;
Diversity;
Endophytic actinomycetes;
Medicinal plants;
PCR screening
- MeSH:
Actinobacteria;
chemistry;
classification;
isolation & purification;
metabolism;
Biodiversity;
China;
Endophytes;
chemistry;
classification;
isolation & purification;
metabolism;
Molecular Sequence Data;
Phylogeny;
Plants, Medicinal;
classification;
microbiology;
Secondary Metabolism
- From:
Chinese Journal of Natural Medicines (English Ed.)
2015;13(12):942-953
- CountryChina
- Language:English
-
Abstract:
The present study was designed to determine the taxonomic diversity and metabolic activity of the actinomycetes community, including 13 traditional medicinal plants collected in Sichuan province, China, using multiple approaches such as morphological and molecular identification methods, bioactivity assays, and PCR screening for genes involved in antibiotics biosynthesis. 119 endophytic actinomycetes were recovered; 80 representative strains were chosen for 16S rRNA gene partial sequence analyses, with 66 of them being affiliated to genus Streptomyces and the remaining 14 strains being rare actinomycetes. Antimicrobial tests showed that 12 (15%) of the 80 endophytic actinomycetes displayed inhibitory effects against at least one indicator pathogens, which were all assigned to the genus Streptomyces. In addition, 87.5% and 58.8% of the isolates showed anticancer and anti-diabetic activities, respectively. Meanwhile, the anticancer activities of the isolates negatively correlated with their anti-diabetic activities. Based on the results of PCR screening, five genes, PKS-I, PKS-II, NRPS, ANSA, and oxyB, were detected in 55.0%, 58.8%, 90.0%, 18.8% and 8.8% of the 80 actinomycetes, respectively. In conclusion, the PCR screening method employed in the present study was conducive for screening and selection of potential actinomycetes and predicting potential secondary metabolites, which could overcome the limitations of traditional activity screening models.
