Comparative analysis of chemical constituents, antimicrobial and antioxidant activities of ethylacetate extracts of Polygonum cuspidatum and its endophytic actinomycete, Streptomyces sp. A0916.
10.1016/S1875-5364(16)60004-3
- Author:
Lei WANG
1
,
2
;
Peng QIU
1
,
2
;
Xiu-Feng LONG
3
;
Shuai ZHANG
3
;
Zhi-Gang ZENG
4
;
Yong-Qiang TIAN
1
,
5
Author Information
1. Key laboratory of Leather Chemistry and engineering, Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, China
2. Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, China.
3. Key laboratory of Leather Chemistry and engineering, Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, China.
4. Sichuan Industrial Institute of Antibiotics, Chengdu 610052, China.
5. Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, China. Electronic address: yqtian@scu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Antimicrobial activity;
Antioxidant activity;
Endophytic actinomycete;
Ethylacetate extracts;
GC/MS;
Polygonum cuspidatum
- MeSH:
Anti-Infective Agents;
chemistry;
isolation & purification;
pharmacology;
Antioxidants;
chemistry;
isolation & purification;
pharmacology;
Bacteria;
drug effects;
Fallopia japonica;
chemistry;
microbiology;
Fungi;
drug effects;
Plant Extracts;
chemistry;
isolation & purification;
pharmacology;
Streptomyces;
chemistry;
classification;
genetics;
isolation & purification
- From:
Chinese Journal of Natural Medicines (English Ed.)
2016;14(2):117-123
- CountryChina
- Language:English
-
Abstract:
The present study investigated the chemical composition of ethylacetate extracts from an endophytic actinomycete Streptomyces sp. A0916 and its host Polygonum cuspidatum. A comparative analysis of the antimicrobial and antioxidant properties of the extracts was also conducted. 32 compounds of P. cuspidatum and 23 compounds of Streptomyces sp. A0916 were isolated and identified by GC/MS. Antimicrobial activities of the extracts were evaluated using eight microbial strains (3 Gram-positive bacteria, 3 Gram-negative bacteria, and 2 fungi). The Streptomyces sp. A0916 extracts showed a wide range of antimicrobial activities and presented greater antimicrobial effectiveness than the P. cuspidatum extracts. The minimum inhibitory concentration (MIC) of Streptomyces sp. A0916 extracts against the ampicillin-resistant strain Enterococcus faecium SIIA843 was 32 μg·mL(-1). Furthermore, the extracts had greater antimicrobial effect against Gram-positive bacteria than Gram-negative bacteria. Finally, the antioxidant activity of the Streptomyces sp. A0916 extracts was equal to that of the P. cuspidatum extracts. In conclusion, our results suggest that the endophytic actinomycetes of the medicinal plants are an important source of bioactive substances.
