Rapid separation and identification of multiple constituents in Danhong Injection by ultra-high performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight tandem mass spectrometry.
10.1016/S1875-5364(16)60008-0
- Author:
Qun-Qun ZHANG
1
;
Xin DONG
1
;
Xin-Guang LIU
1
;
Wen GAO
1
;
Ping LI
2
;
Hua YANG
3
Author Information
1. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
2. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: liping2004@126.com.
3. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: yanghuacpu@126.com.
- Publication Type:Journal Article
- Keywords:
Danhong Injection;
Flos Carthami tinctorii;
Mass spectrometry;
Radix Salviae Miltiorrhizae;
TCM;
UHPLC-ESI-QTOF/MS
- MeSH:
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
chemistry;
isolation & purification;
Spectrometry, Mass, Electrospray Ionization;
methods;
Tandem Mass Spectrometry;
methods
- From:
Chinese Journal of Natural Medicines (English Ed.)
2016;14(2):147-160
- CountryChina
- Language:English
-
Abstract:
To characterize and identify multiple constituents in Danhong injection (DHI), a fast ultra-high performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QTOF/MS) method was established and validated in the present study. A total of 63 compounds, including 33 phenolic acids, 2 C-glycosyl quinochalcones, 6 flavonoid O-glycosides, 4 iridoid glycosides, 6 organic acids, 5 amino acids, and 3 nucleosides, were identified or tentatively characterized. In conclusion, the UHPLC-ESI-QTOF/MS method is useful and efficient for in-depth structural elucidation of chemical compounds in complex matrices of herbal medicines such as DHI.
