Rapid screening and identification of sesquiterpene lactones in Kudiezi injection based on high-performance liquid chromatography coupled with linear ion trap-orbitrap mass spectrometry.
10.1016/S1875-5364(18)30042-6
- Author:
Rong-Rong LIU
1
;
Xiu-Ping ZHANG
1
;
Fang WANG
1
;
Zhan-Peng SHANG
1
;
Fei WANG
1
;
Ying LIU
2
;
Jian-Qiu LU
3
;
Jia-Yu ZHANG
4
Author Information
1. School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100029, China.
2. Beijing Research Institution of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
3. Library of Beijing University of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China. Electronic address: lujq@vip.sina.com.
4. Beijing Research Institution of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China. Electronic address: zhangjiayu0615@163.com.
- Publication Type:Journal Article
- Keywords:
Characteristic fragmentation pathways;
HPLC-LTQ-Orbitrap;
Kudiezi injection;
Sesquiterpene lactones
- MeSH:
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
chemistry;
Isomerism;
Lactones;
chemistry;
Sesquiterpenes;
chemistry;
Tandem Mass Spectrometry;
methods
- From:
Chinese Journal of Natural Medicines (English Ed.)
2018;16(2):150-160
- CountryChina
- Language:English
-
Abstract:
Sesquiterpene lactones are considered as the major active compounds in Kudiezi injection in virtue of their special structures and activities. Herein, an analytical method was developed for rapid screening and identification of sesquiterpene lactones in Kudiezi injection using high-performance liquid chromatography coupled with linear ion trap-orbitrap mass spectrometry (HPLC-LTQ-Orbitrap) in negative ion mode. First, two sesquiterpene lactone reference standards were analyzed to obtain their characteristic ESI-MS/MS fragmentation patterns. Second, based on extracted ion chromatography (EIC) data-mining method and characteristic fragmentation pathways analysis, sesquiterpene lactones in Kudiezi injection were rapidly screened and identified. Finally, an important parameter Clog P was adopted to discriminate the isomers of sesquiterpene lactones. As a result, 50 sesquiterpene lactones were characterized, including 9 sesquiterpene lactone aglycones, 39 sesquiterpene lactone glycosides, and 2 amino acid-sesquiterpene lactone conjugates. Among them, 13 compounds were tentatively identified as new compounds. The results demonstrated that the established method would be a rapid, effective analytical tool for screening and identification of sesquiterpene lactones in the complex system of natural medicines.
