Qualitative detection of ginsenosides in brain tissues after oral administration of high-purity ginseng total saponins by using polyclonal antibody against ginsenosides.
10.1016/S1875-5364(18)30045-1
- Author:
Yu-Nan ZHAO
1
;
Xiao SHAO
2
;
Liu-Feng OUYANG
3
;
Lin CHEN
2
;
Ling GU
4
Author Information
1. Laboratory of Pathological Sciences, College of Medicine and Life, Nanjing University of Chinese Medicine, Nanjing 210046, China. Electronic address: zhaoyunan-js@163.com.
2. Laboratory of Pathological Sciences, College of Medicine and Life, Nanjing University of Chinese Medicine, Nanjing 210046, China.
3. Laboratory of Pathological Sciences, College of Medicine, Yan'an University, Yan'an 716000, China.
4. Research Center, Basic Medical College, Nanjing University of Chinese Medicine, Nanjing 210046, China.
- Publication Type:Journal Article
- Keywords:
Blood–brain barrier;
Ginseng;
Ginsenoside;
Immunostaining;
Mass spectrometry
- MeSH:
Administration, Oral;
Animals;
Antibodies;
analysis;
Blood-Brain Barrier;
metabolism;
Brain;
metabolism;
Brain Chemistry;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
administration & dosage;
analysis;
metabolism;
Ginsenosides;
administration & dosage;
analysis;
metabolism;
Male;
Mice;
Mice, Inbred C57BL;
Panax;
chemistry;
Rats;
Rats, Sprague-Dawley;
Tandem Mass Spectrometry
- From:
Chinese Journal of Natural Medicines (English Ed.)
2018;16(3):175-183
- CountryChina
- Language:English
-
Abstract:
Given the limited studies and conflicting findings, the transport character of ginsenosides crossing the blood-brain barrier (BBB) remains unclear. The present study was designed to qualitatively determine the distribution of ginsenosides in brain tissues after oral administration of ginseng total saponins, using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) combined with immunohistochemistry. In brain tissue homogenates, ginsenoside Rg1 was detectable and no other ginsenosides or their metabolites were found. No ginsenosides were detected in cerebrospinal fluid. Immunohistochemistry staining of brain tissue sections by using anti-ginsenoside polyclonal antibodies revealed the localization of ginsenosides in brain tissues. Furthermore, immunofluorescence double staining revealed that ginsenosides widely existed in vascular endotheliocytes and astrocytes, and in few neurons. These results indicated that Rg1 was the main component that entered the brain after oral administration of ginseng total saponins and that ginsenosides could cross the BBB, although the transport capability of ginsenosides through the BBB may be poor.
