Constituent and effects of polysaccharides isolated from Sophora moorcroftiana seeds on lifespan, reproduction, stress resistance, and antimicrobial capacity in Caenorhabditis elegans.
10.1016/S1875-5364(18)30055-4
- Author:
Yuan ZHANG
1
;
Dan-Yang MI
1
;
Jin WANG
1
;
Yan-Ping LUO
1
;
Xu YANG
1
;
Shi DONG
1
;
Xing-Ming MA
2
,
3
;
Kai-Zhong DONG
4
Author Information
1. Department of Immunology, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China.
2. Department of Immunology, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China
3. Key Lab of Preclinical Study for New Drugs of Gansu Province, Lanzhou 730000, China. Electronic address: maxm@lzu.edu.cn.
4. Department of Microbiology, Medical College, Northwest University for Nationalities, Lanzhou 730000, China. Electronic address: dkz@xbmu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Anti-infection;
Caenorhabditis elegans;
Polysaccharide;
Reproduction;
Sophora moorcroftiana;
Stress resistance
- MeSH:
Animals;
Caenorhabditis elegans;
drug effects;
physiology;
Longevity;
drug effects;
Plant Extracts;
chemistry;
isolation & purification;
pharmacology;
Polysaccharides;
chemistry;
isolation & purification;
pharmacology;
Reproduction;
drug effects;
Seeds;
chemistry;
Sophora;
chemistry;
Stress, Physiological;
drug effects
- From:
Chinese Journal of Natural Medicines (English Ed.)
2018;16(4):252-260
- CountryChina
- Language:English
-
Abstract:
Sophora moorcroftiana (S. moorcroftiana) is an endemic leguminous dwarf shrub in Tibet, China. Decoctions of the seeds have been used in Chinese folk medicine for dephlogistication, detoxication, and infectious diseases. The present study aimed to investigate the constituent and biological effects of polysaccharides from S. moorcroftiana seeds in Caenorhabditis elegans (C. elegans). Polysaccharides from S. moorcroftiana seeds (SMpol) were extracted with 60% ethanol and constituent was analyzed by GC-MS. SMpol was composed of glucose, galactose and inositol in the molar ratio of 35.7 : 1.3 : 17.0. Synchronized worms were treated with SMpol and then lifespan, motility, reproduction, stress resistance and antimicrobial activity were examined. Compared with the control group, the lifespan was increased to the average of 27.3 days and the number of laying eggs showed a 1.3-fold increase in nematodes treated with SMpol (4 mg·mL). In SMpol (4 mg·mL) treated worms, there was a 1.1-fold increase in 24-h survival of acute heat stress and a 1.6-fold increase in 2-h survival of oxidative stress The colonization of the bacteria in the SMpol treated nematode was significantly lower than that of the untreated group by 68.3%. In vivo studies showed SMpol significantly extended the life span, improved reproduction, increased stress resistance and antimicrobial capacity of C. elegans. In conclusion, those results indicated that the polysaccharides from S. moorcroftiana seeds were involved in a variety of biological activities leading to its modulatory effects on C. elegans which may be developed as a natural supplement agent.
